Showing papers by "James D. Young published in 1989"
TL;DR: The rank order of potency of tachykinins and related analogues as inhibitors of [125I]BHSP binding to astrocytoma membranes and intact cells correlated with their relative abilities to stimulate uridine incorporation into nucleic acid.
56 citations
TL;DR: When reconstituted into proteoliposomes, the human erythrocyte nucleoside transporter catalysed nitrobenzylthioguanosine (NBTGR)-sensitive zero-trans influx of three different nucleosides at broadly similar rates (inosine, uridine greater than adenosine).
5 citations
01 Jan 1989
5 citations
TL;DR: Binding of 125I‐insulin to human astrocytoma cells was time dependent, reaching equilibrium after 1 h at 22°C with equilibrium binding corresponding to 2.2 fmol/mg protein: this represents approximately 2,000 occupied binding sites per cell.
Abstract: Binding of [125I]monoiodoinsulin to human astrocytoma cells (U-373 MG) was time dependent, reaching equilibrium after 1 h at 22 degrees C with equilibrium binding corresponding to 2.2 fmol/mg protein: this represents approximately 2,000 occupied binding sites per cell. The t1/2 of 125I-insulin dissociation at 22 degrees C was 10 min; the dissociation rate constant of 1.1 X 10(-2) s-1 was unaffected by a high concentration of unlabeled insulin (16.7 microM). Porcine insulin competed for specific 125I-insulin binding in a dose-dependent manner and Scatchard analysis suggested multiple affinity binding sites (higher affinity Ka = 4.4 X 10(8) M-1 and lower affinity Ka = 7.4 X 10(6) M-1). Glucagon and somatostatin did not compete for specific insulin binding. Incubation of cells with insulin (0.5 microM) for 2 h at 37 degrees C increased [2-14C]uridine incorporation into nucleic acid by 62 +/- 2% (n = 3) above basal. Cyclic AMP, in the absence of insulin, also stimulated nucleoside incorporation into nucleic acid [65 +/- 1% (n = 3)] above basal. Preincubation with cyclic AMP followed by insulin had an additive effect on nucleoside incorporation [160 +/- 4% (n = 3) above basal]. Dipyridamole (50 microM), a nucleoside transport inhibitor, blocked both basal and stimulated uridine incorporation. These studies confirm that human astrocytoma cells possess specific insulin receptors with a demonstrable effect of ligand binding on uridine incorporation into nucleic acid.
5 citations
TL;DR: This paper presents a meta-analyses of the prophylactic and cellular defences of the immune system in response to the presence of canine coronavirus, which shows clear patterns of inflammation in both the Hr.
Abstract: Boobis, A. R., Brodie, M. J., Kahn, G. C., Fletcher, D. R., Saunders, J. H. & Davies. D. S. ( 1980) Hr. J . C’lin. I’hurmucol. 9, 1 1 19 Grivas. S. (1988) J . C’hem. Kes. ( S ) 84 Maron, D. M. & Ames, B. N. ( 1983) Mutut. Re.\. 113, 173-2 I5 McCalla, D. R., Reuvers, A. & Kaiser, C. ( 1 970) J . Racteriol. 104, 1126-1 134 Murray, B. P., Boobis, A. R., de la Torre. R., Segura, J. & Davies, D. S. ( 1 988u) Riochem. Soc. Trans. 16,620-62 1 Murray, S., Gooderham, N. J., Boobis, A. R. & Davies, D. S. ( 1 988h) Carcinogenesis 9.321 -325 Rosenkranz, H. S., McCoy, E. C., Sanders, D. R., Butler, M., Kiriazides, D. K. & Mermelstein, R. (1980) Science 209,
2 citations
2 citations