Showing papers by "Jean Weissenbach published in 1999"
TL;DR: The BACs are shown how they provide molecular links for understanding human genomic duplications, meiosis, and evolution, as well as reagents for conducting genome-wide prenatal diagnosis at the molecular level and for detecting gene candidates associated with novel cancer breakpoints.
Abstract: Translating problems of human disease into the language of the human genome requires a unified resource that bridges DNA sequence through chromosome bands. Such a resource must link the three types of linear arrays that represent the human genome: database arrays (genetic and physical maps and ultimately DNA sequence), chromosome bands visible in single cells, and ordered clone arrays. Genome maps have been previously either STS-based, with marker order obtained using a combination of STS-content of large insert yeast artificial chromosome (YAC) clones, radiation hybrid (RH) mapping, and genetic mapping (Hudson et al. 1995; Deloukas et al. 1998), or BAC-based, with order obtained at 2–6 Mb through high resolution mapping by fluorescence in situ hybridization (FISH) with respect to human chromosome landmarks (Korenberg et al. 1992). During the course of these efforts, a strategy to integrate these maps was established. BACs are well suited for a permanent FISH-mapped and integrated clone resource in that they represent a stable and easily manipulated form of cloned DNA and produce bright, well defined signals on metaphase and interphase chromosome preparations (Korenberg and Chen 1995). We now report the construction of a genome-wide array of bacterial artificial chromosomes (BACs) that is integrated with the cytogenetic, genetic, and STS maps and characterized for homology to the remainder of the human genome by FISH.
65 citations