J
Jesse J. Salk
Researcher at University of Washington
Publications - 65
Citations - 3262
Jesse J. Salk is an academic researcher from University of Washington. The author has contributed to research in topics: Medicine & Biology. The author has an hindex of 19, co-authored 49 publications receiving 2658 citations. Previous affiliations of Jesse J. Salk include Fred Hutchinson Cancer Research Center.
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Detection of ultra-rare mutations by next-generation sequencing
Michael W. Schmitt,Scott R. Kennedy,Jesse J. Salk,Edward J. Fox,Joseph Hiatt,Lawrence A. Loeb +5 more
TL;DR: It is determined that Duplex Sequencing has a theoretical background error rate of less than one artifactual mutation per billion nucleotides sequenced and that detection of mutations present in only one of the two strands of duplex DNA can be used to identify sites of DNA damage.
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Detecting ultralow-frequency mutations by Duplex Sequencing
Scott R. Kennedy,Michael W. Schmitt,Edward J. Fox,Brendan F. Kohrn,Jesse J. Salk,Eun Hyun Ahn,Marc J. Prindle,Kawai J. Kuong,Jiang Cheng Shen,Rosa Ana Risques,Lawrence A. Loeb +10 more
TL;DR: A detailed protocol for efficient DS adapter synthesis, library preparation and target enrichment, as well as an overview of the data analysis workflow are provided.
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Enhancing the accuracy of next-generation sequencing for detecting rare and subclonal mutations.
TL;DR: The technical challenges in characterizing subclonal variants using conventional NGS protocols and the recent development of error correction strategies are described, both computational and experimental, including consensus sequencing of single DNA molecules.
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Ultra-Sensitive Sequencing Reveals an Age-Related Increase in Somatic Mitochondrial Mutations That Are Inconsistent with Oxidative Damage
TL;DR: The results argue against oxidative damage being a major driver of aging and suggest that replication errors by DNA polymerase γ and/or spontaneous base hydrolysis are responsible for the bulk of accumulating point mutations in mtDNA.
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Mutational Heterogeneity in Human Cancers: Origin and Consequences
TL;DR: Detailed information is gathered on the nucleotide sequence changes in a number of human cancers to help identify key rate-limiting genes for tumor growth that could serve as potential targets for directed therapies.