Jessica L. Spears

TL;DR: The 2-thiouridine at the wobble position of certain bacterial and eukaryotic tRNAs enhances aminoacylation kinetics, assists proper codon-anticodon base pairing at the ribosome A-site, and prevents frameshifting during translation.

TL;DR: In this paper, the authors used Monte Carlo and self-consistent mean field (SVM) to optimize the peptide sequence in silico, while fluorescence assays were developed and conducted to verify the in-silico results and elucidate a 15-amino acid signature.

TL;DR: In this paper, the anticodon stem and loop of the ASL(Arg1,2) constructs were analyzed by spectroscopy and codon binding assays, and it was shown that the solution structures of these structures were nearly identical.

TL;DR: Three of six arginine codons (CGU, CGC and CGA) are decoded by two Escherichia coli tRNAArg isoacceptors and the adoption of a spatially broad inosine-adenosine base pair at the wobble position of the codon cannot be maintained simultaneously with the canonical ASL U-turn motif.

TL;DR: It is found that Bacillus subtilis BS50 exhibits a robust preclinical safety profile commensurate with commercial probiotic strains and likely poses no significant health risk to humans.