Showing papers by "Jian Ni published in 2015"
TL;DR: A systematic review of the literature on Polygonum multiflorum was performed using several resources, including classic books on Chinese herbal medicine and various scientific databases, to provide comprehensive information on the botany, traditional uses, phytochemistry, pharmacological research and toxicology of this plant.
277 citations
TL;DR: The analysis methods suggest that PM toxicity may be associated with anthraquinone, emodin-O-(malonyl)-hex, emoda-O-glc, emodein, emmodin-8-O -glc), emod in-O"-acetyl-hex, and emodIn-O’-hex-sulphate.
71 citations
TL;DR: Tandem high-resolution mass spectrometry (THRMS) is an analytical technique that has arisen in recent years and is now widely used in pharmaceutical research and development (RD for example, for the identification of constituents in herbs and formulae, pharmacokinetics, omics, and drug degradation), food safety, environmental contamination and other research fields as mentioned in this paper.
Abstract: Tandem high-resolution mass spectrometry (THRMS) is an analytical technique that has arisen in recent years and is now widely used in pharmaceutical research and development (RD for example, for the identification of constituents in herbs and formulae, pharmacokinetics, omics, and drug degradation), food safety, environmental contamination and other research fields. Time of Flight (TOF) and Orbitrap are the most widely used mass analysers in THRMS, and the technical specifications vary among the different types of THRMS and even among the different manufacturers for a given type of analyser. In this article, we review the principle and functional characteristics of different types or models for THRMS and provide a brief description of its applications in the medical research, food safety, and environmental protection fields.
26 citations
TL;DR: A simple, rapid method using resolution liquid chromatography coupled with a triple quadruple mass spectrometry to identify and quantify the major bioactive constituents in RPM was demonstrated to be simple, sensitive and reproducible.
Abstract: Radix polygoni multiflori (RPM) has antioxidative, anti-aging, liver-protective and antihuman cytomegalovirus activity. It has been proved to be hepatotoxic. Considering multiple ingredients to control RPM quality is essential. The aim of this study was to establish a simple, rapid method using resolution liquid chromatography coupled with a triple quadruple mass spectrometry to identify and quantify the major bioactive constituents in RPM. The method was applied to analyze 14 marker batches from manufacturers from the same province. The ultrasonic extracts of all samples were determined by LC-MS/MS, and assessed by hierarchical cluster analysis. The proposed method was applied to analyze 21 batches of samples with acceptable linearity (R(2) , 0.9930-0.9998), precision (relative standard deviation, RSD, 0.45-4.73%) repeatability (RSD, 1.14-9.41%), stability (RSD, 1.29-12.88%) and recovery (RSD, 1.80-12.15%) of the 14 compounds. Furthermore, the hierarchical cluster analysis was applied to classify 21 samples on the basis of characteristics of the 14 compound markers. The developed method was demonstrated to be simple, sensitive and reproducible, and has significant importance and comprehensive evaluation for quality control of RPM and related preparations. Hierarchical cluster analysis clearly indicated that the RPM from the same province was similar, whereas samples of RPM from different provinces were significantly different.
19 citations
TL;DR: This plant possesses diverse pharmacological activities, such as anti-inflammatory, antitumor, antibacterial, antiviral, cytotoxic, as well as insecticidal activities.
Abstract: Ajuga decumbens Thunb is a member of Labiatae family and widespread in China, Korea and Japan. This plant possesses diverse pharmacological activities, such as anti-inflammatory, antitumor, antibacterial, antiviral, cytotoxic, as well as insecticidal activities. Several compounds have been isolated from A. decumbens, which display a wide spectrum of biological and pharmacological activities. Hence, it would be useful to review current literature for available pharmacological activities of the plant as well as its active ingredients.
16 citations
TL;DR: An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry method was established and was successfully utilized for screening and characterizing the ingredients and metabolites of RPM.
Abstract: An ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry method was established to detect as many constituents in rat plasma as possible after oral administration of Radix polygoni multiflori (RPM) extract. A C18 column (150 × 2.0 mm, 4 µm) was adopted to separate the samples, and mass spectra were acquired in negative modes. The fingerprints of RPM extract were established, resulting in 39 components being detected. Among these compounds, 29 were identified by comparing the retention times and mass spectral data with those of reference standards and relevant references, and eight compounds were separated and detected in RPM for the first time. In vivo, 23 compounds were observed in dosed rat plasma, 16 of 23 compounds were indicated as prototype components of RPM, and seven compounds were predicted to be metabolites of RPM. A high-speed and sensitive method was developed and was successfully utilized for screening and characterizing the ingredients and metabolites of RPM. Copyright © 2015 John Wiley & Sons, Ltd.
12 citations
TL;DR: A selective and rapid ultra-performance liquid chromatography method with tandem mass spectrometric (UPLC-MS/MS) detection for simultaneous determination of typhaneoside and isorhamnetin-3-O-neohesperidoside in rat plasma was developed and validated, which was applied to the pharmacokinetic study of Pollen Typhae extract.
Abstract: For the first time, a selective and rapid ultra-performance liquid chromatography method with tandem mass spectrometric (UPLC-MS/MS) detection for simultaneous determination of typhaneoside and isorhamnetin-3-O-neohesperidoside in rat plasma was developed and validated, which was applied to the pharmacokinetic study of Pollen Typhae extract. The separation was carried out on an ACQUITY UPLC(TM) BEH C18 column with gradient elution using mobile phase including acetonitrile and water (containing 0.1% formic acid). The flow rate was 0.4 mL/min. The detection was conducted by means of electrospray ionization mass spectrometry in negative ion mode with multiple reaction monitoring. The assays were linear over the concentration range of 0.5-100 ng/mL, and the lower limit of quantification was 0.5 ng/mL for typhaneoside and isorhamnetin-3-O-neohesperidoside. The method was validated in terms of intra- and interday precision (<9.37%), accuracy (within ±10.91%), linearity, specificity and stability, and has been successfully applied to a pharmacokinetic study of Pollen Typhae extract in rats after oral administration.
10 citations
TL;DR: Results indicated that, compare to improve the solubility, it is more important to maintain and prolong the T-OA dissolved status, for improvement of the in-vivo absorption.
Abstract: The goal of this study was to enhance the absorption of a new water-insoluble antitumor lead compound, T-OA (3β-hydroxyolea-12-en-28-oic acid-3, 5, 6-trimethylpyrazin-2-methyl ester). Early-stage preparation discovery concept (EPDC) was employed in this study. Based on this concept, a microemulsion system was chosen as the method of improving bioavailability. The solubility of T-OA was checked in different oils, surfactants and cosurfactants. Ternary phase diagrams were constructed to evaluate the microemulsion domain. Developed high-performance liquid chromatography method was used to determine drug content. The transparent o/w microemulsion formulation composed of oleic acid (oil), Tween 80 (surfactant), ethanol (co-surfactant) and water enhanced the solubility of T-OA up to 20 mg/mL. It was characterized in terms of appearance, content, viscosity, zeta potential, conductivity, morphology and particle size. The particle size distribution, viscosity, conductivity and zeta potential were found to ...
9 citations
TL;DR: In this article, the authors investigated the stability of forsythiaside in rat plasma and found that it was stable at temperatures greater than 37 °C and volatile at −20 °C.
Abstract: Forsythiaside is very unstable and can be affected by different conditions. Studying the stability of forsythiaside in vivo would greatly aid in accurately measuring it and evaluating its pharmacokinetics. No thorough study has been performed examining its stability during bio-sample pretreatment processing. This study is the first to completely investigate forsythiaside stability in plasma sample pretreatment processing. The time and temperature were the two key factors that determined forsythiaside stability in plasma pretreatment. Spiked plasma samples stored at 25 °C for 0.5 h or at 0 °C for 4 h were stable, and plasma samples vortexed with an internal standard (IS) and ethyl acetate were stable at 25 °C for 2 h or −20 °C for 4 h. An organic phase that was evaporated at 25 °C had high stability at temperatures greater than 37 °C. The storage time of spiked plasma and the vortex-mixed samples exerted a tremendous influence on the stability of forsythiaside. The storage, centrifugation and evaporation temperatures played indispensable roles in forsythiaside stability. During sample preparation, the reconstitution and injection times satisfied the requirements necessary for measuring forsythiaside in a pharmacokinetic study. Treating the plasma samples under the conditions investigated in this study will ensure that the data and parameters will be stable and controllable for a pharmacokinetic study and will be suitable for measuring forsythiaside in rat plasma. Moreover, this study will be a reference for both stability in other bio-matrices and pharmacokinetic studies in further research.
7 citations
TL;DR: In this article, a rapid and specific liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous analysis of P-hydroxybenzaldehyde, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, emodin-8-O-,β-D-Glucopyranoside, and emoderin in rat plasma, and it was applied to the pharmacokinetics (PK) studies of the four compounds from the herb
Abstract: A rapid and specific liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for the simultaneous analysis of P-hydroxybenzaldehyde, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, emodin-8-O-β-D-glucopyranoside, and emodin in rat plasma, and it was applied to the pharmacokinetics (PK) studies of the four compounds from the herb Polygonum multiflorum. The analysis was carried out on a Phenomenex Hydro-RP C18 (150 × 2.0 mm, 4 μm) reversed-phase column by gradient elution with a flow rate of 0.4 mL min−1. All of the analytes including internal standards (I.S.) were monitored by selected reaction monitoring with an electrospray ionization source. Linear responses were obtained for P-hydroxybenzaldehyde, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, emodin-8-O-β-D-glucopyranoside, and emodin ranging from 1 to 1000 ng mL−1. The intra-day and inter-day precisions (RSD) were less than 8.61% and 8.75%, respectively. The extraction recovery ranged from 77.00 ± 5.54 to 91.30 ± 2.96, and the I.S. was 85.82 ± 3.58%. Stability studies showed that the accuracies of P-hydroxybenzaldehyde, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, emodin-8-O-β-D-glucopyranoside, and emodin ranged from 94.66 ± 3.54 to 106.84 ± 6.91; the matrix effects ranged from 92.14 ± 3.63 to 103.98 ± 8.71. The validated method was successfully used to determine the concentration–time profiles of P-hydroxybenzaldehyde, 2,3,5,4′-tetrahydroxystilbene-2-O-β-glucoside, emodin-8-O-β-D-glucopyranoside, and emodin.
5 citations
TL;DR: In this article, the activation energy of 3,6′-disin apoylsucrose, tenuifoliside A, 10uifolside B and tenuoliside C in neutral aqueous solution was calculated to be 52.00, 42.25, 39.64, and 45.50 kJ mol−1, respectively.
Abstract: 3,6′-Disin apoylsucrose, tenuifoliside A, tenuifoliside B and tenuifoliside C are the major pharmacologically active ingredients in Radix Polygalae. The hydrolytic kinetics and degradation mechanism of these compounds were studied using high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS). The influence of temperature and pH on the stability of these compounds was investigated. The activation energy of 3,6′-disin apoylsucrose, tenuifoliside A, tenuifoliside B and tenuifoliside C in neutral aqueous solution (pH 6.8) was calculated to be 52.00, 42.25, 39.64, and 45.50 kJ mol−1, respectively. These four oligosaccharide esters were more susceptible to degradation under alkaline conditions. The minimum stability of 3,6′-disin apoylsucrose, tenuifoliside A, tenuifoliside B and tenuifoliside C were found at pH 10.8, and t1/2 was 0.21, 0.02, 0.17 and 0.03, respectively. The degradation products of these compounds were identified by UHPLC-Q-TOF/MS, and the degradation mechanisms were thought to be hydrolysis and isomerization, such as cis-trans isomerism, keto–enol tautomerism and optical isomerism.
TL;DR: It was found that intranasal administration of ginsenoside Rd exhibited good brain-targeting and the method was validated in terms of specificity, linearity, intra- and inter-day precision, dilution integrity, recovery, matrix effects and stability.
Abstract: Ginsenoside Rd was shown to have protective effects against several injuries and efficient for the treatment of acute ischemic stroke. Some research studies of ginsenoside Rd in the past mainly focused on the pharmacokinetics after intravenous and oral administration. However, we still lack some basic knowledge about the plasma pharmacokinetics and brain distribution of ginsenoside Rd by any other route, such as intranasal administration. It was found that intranasal administration exhibited good brain-targeting. In this study, a sensitive LC-MS/MS method was developed and validated for the determination of ginsenoside Rd in rat plasma and brain tissue. Detection was performed on an ACQUITY UPLC™ BEH C18 column using gradient elution with a flow rate of 0.2 mL min−1. Mass spectrometry was operated in selected reaction monitoring mode using a negative electrospray ionization interface. The method was linear over the concentration range of 1.0–1000 ng mL−1, and the lower limit of quantification was 1.0 ng mL−1 for ginsenoside Rd. The method was validated in terms of specificity, linearity, intra- and inter-day precision (<12.39%), accuracy (within ±10.1%), dilution integrity, recovery, matrix effects and stability, and has been successfully applied to the pharmacokinetic study of ginsenoside Rd in rats after intranasal administration and evaluation of the brain targeting of ginsenoside Rd.
TL;DR: Wang et al. as discussed by the authors used Locally Weighted Regression (LWR) to predict the total flavonoids concentration of Chinese herb fried with bran in its extraction process.