The invention discloses a wheat spike density QTL linked HRM (high resolution melting) molecular marker and application thereof and provides a wheat spike density QTL Qsd.sau-7A linked molecular marker. The molecular marker is 0C32-715, and a nucleotide sequence is shown as SEQ ID NO:1. According to detection and analysis, the molecular marker is capable of closely tracking wheat spike density QTL to predict spikelet dense insertion characteristics, and then selecting and breeding of a high-spike-density variety (strain) can be realized through molecular assisted selection. The invention further provides application of the molecular marker 0C32-715 to wheat breeding. By adoption of the method, accuracy in wheat spike density prediction can be improved in a certain range to make it convenient for quickly screening out the wheat variety or strain with spike density value increasing QTL for breeding, and accordingly a wheat high-yield variety selection process can be greatly accelerated.

Wheat spike density QTL linked HRM (high resolution melting) molecular marker and application thereof

The invention provides an SSR molecular marker of wheat glume tomentum gene Hg linkage and an application thereof. The genetic distance between the molecular marker saufc2 and a glume tomentum gene Hg is 1.7 cM, and the marker is obtained through amplification of a primer of which the nucleotide sequence is shown as SEQ ID No.1-2. The linkage between the molecular marker saufc2 and the wheat glume tomentum gene Hg can be used for tracking wheat glume tomentum genes, assisting in detecting whether wheat has the glume tomentum character or not and conducting molecular assisted selection.

SSR molecular marker of wheat glume tomentum gene Hg linkage and application thereof

The invention provides a molecular marker R207 coseparated with a few-tillering gene Ltn3 of wheat, wherein the marker R207 is located on a long arm of a 2D chromosome of wheat, and the nucleotide sequence is shown as in SEQ ID NO:1. The molecular marker R207 is coseparated with the few-tillering gene Ltn3 and can be used to the marker-assisted selection; and the detection result shows that the molecular marker can accurately track the few-tillering gene of wheat and predicts the tillering feature of the wheat, so that molecular designing and breeding can be conveniently conducted. The molecular marker and the detection method improve the accuracy of tillering predication, improve the success rate of breeding of a special plant, and accelerate the realization of the target of increasing per unit yield of the wheat.

Molecular marker R207 coseparated with wheat few-tillering gene Ltn3 and application of molecular marker R207

The invention discloses a molecular marker SSR52 which is closely linked with wheat few-tillering gene Ltn3, wherein the nucleotide sequence of the molecular marker SSR52 is represented as the SEQ ID No.1. Genetic distance between the molecular marker and the wheat few-tillering gene Ltn3 is 0.3 cM. A test result proves that the molecular marker can accurately trace the wheat few-tillering gene and predict tillering characters of wheat, thereby further carrying molecular-designing breeding conveniently. The invention also discloses a method of identifying the molecular marker of the wheat few-tillering gene Ltn3. By means of the method, accuracy of tillering prediction can be increased, success rate of specific plant type breeding can be increased, and achievement of an object of increasing per unit area yield of wheat is accelerated.

Molecular marker SSR52 of wheat few-tillering gene Ltn3 and application thereof

The invention relates to the field of barley molecular breeding, and in particular discloses a molecular marker HRM7 of barley grain length gene LkI2 and application of the molecular marker. The nucleotide sequence of the molecular marker HRM7, which is closely linked with the barley grain length gene LkI2, is as shown in SEQ ID No.1. Upon detection and analysis, the molecular marker can accurately track the barley grain length gene and predict the grain length traits of the barley, so as to facilitate molecular designing and breeding. The invention also discloses a method for identifying the molecular marker of the barley grain length gene LkI2; by virtue of the method provided by the invention, the accuracy of grain length prediction is enhanced, so that barley varieties or lines having enhanced grain length QTL can be conveniently and rapidly screened out; and the process of selecting high-yield barley varieties can be greatly accelerated.

Jiang Qiantao

Papers

Molecular marker SSR52 of wheat few-tillering gene Ltn3 and application thereof

Molecular marker HRM7 of barley grain length gene LkI2 and application of molecular marker