Jing Xie

TL;DR: An environmentally sustainable production platform for a variety of correctly folded cyclic disulfide-rich peptides with enhanced yields.

TL;DR: In this article, a protocol for cyclic peptide precursors in Pichia pastoris that undergo in vitro enzymatic maturation into cyclic disulfide-rich peptides using recombinant asparaginyl endopeptidases (AEPs) is described.

TL;DR: Asparaginyl ligases as discussed by the authors are a class of highly efficient transpeptidases with the capacity to modify proteins bearing only a tripeptide recognition motif, and they have been used for a variety of protein modifications, including N-and C-terminal protein labeling, head-to-tail cyclisation, and protein-protein conjugation.

TL;DR: In this paper , two classes of peptide toxin are identified as responsible for the symptoms of U. ferox stings: Δ-Uf1a is a cytotoxic thionin that causes pain via disruption of cell membranes, while β/δ-uf2a defines a new class of neurotoxin which causes pain and systemic symptoms via modulation of voltage-gated sodium (NaV) channels.

TL;DR: In this paper , the capacity of the Australian native Nicotiana benthamiana plant to produce a structurally identical [T20K]kB1 to that of the synthetic peptide was demonstrated.