J
João Rosa
Researcher at Karolinska Institutet
Publications - 10
Citations - 909
João Rosa is an academic researcher from Karolinska Institutet. The author has contributed to research in topics: Colloidal gold & Recognition sequence. The author has an hindex of 7, co-authored 10 publications receiving 750 citations. Previous affiliations of João Rosa include Universidade Nova de Lisboa.
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Journal ArticleDOI
Noble metal nanoparticles for biosensing applications.
Gonçalo Doria,João Conde,Bruno Veigas,Letícia Giestas,Carina Almeida,Maria Assunção,João Rosa,Pedro V. Baptista +7 more
TL;DR: The use of noble metal nanoparticles for biosensing strategies is reviewed—from synthesis and functionalization to integration in molecular diagnostics platforms, with special focus on those that have made their way into the diagnostics laboratory.
Journal ArticleDOI
Binding to Nanopatterned Antigens is Dominated by the Spatial Tolerance of Antibodies
Alan Shaw,Ian T. Hoffecker,Ioanna Smyrlaki,João Rosa,Algirdas Grevys,Algirdas Grevys,Diane Lynn Bryant Bratlie,Inger Sandlie,Inger Sandlie,Terje E. Michaelsen,Terje E. Michaelsen,Jan Terje Andersen,Jan Terje Andersen,Björn Högberg +13 more
TL;DR: It is found that all the subclasses and isotypes studied bind bivalently to two antigens separated at distances that range from 3 to 17 nm, and considerable differences in spatial tolerance exist between IgM and IgG and between low- and high-affinity antibodies.
Journal ArticleDOI
Gold-nanobeacons for simultaneous gene specific silencing and intracellular tracking of the silencing events.
TL;DR: A single nanostructure can be used to intersect all RNA regulatory pathways while allowing for direct assessment of effective silencing and cell localization via a quantifiable fluorescence signal, making cancer nanotheranostics possible.
Journal ArticleDOI
Gold-nanobeacons for real-time monitoring of RNA synthesis
TL;DR: This biosensor may assist the in vitro evaluation of silencing potential of a given sequence to be later used for in vivo gene silencing and can easily be extended and adapted to situations when real-time quantitative assessment of RNA synthesis and determination of the level of inhibition are required.
Journal ArticleDOI
Nanophotonics for Molecular Diagnostics and Therapy Applications
TL;DR: The uses of nanobiophotonics for molecular diagnostics involving specific sequence characterization of nucleic acids and for gene delivery systems of relevance for therapy strategies and the translation of nanophotonic for theranostics into the clinical setting will be discussed.