John C. Jewett

TL;DR: This tutorial review will summarize the history of this emerging field, as well as recent progress in the development and application of bioorthogonal copper-free click cycloaddition reactions.

TL;DR: A biarylazacyclooctynone (BARAC) that has exceptional reaction kinetics and whose synthesis is designed to be both modular and scalable is described and employed for live cell fluorescence imaging of azide-labeled glycans.

TL;DR: A systematic analysis of the effects of strain and electronics on the reactivity of cyclooctynes with azides through both experimental measurements and computational studies using a density functional theory (DFT) distortion/interaction transition state model suggests a correlation between decreased alkyne bond angle and increasedcyclooctyne reactivity.

TL;DR: A chemical approach for probing PG in vivo via metabolic labeling and bioorthogonal chemistry and click chemistry detection constitute a facile, modular platform that facilitates unprecedented spatial and temporal resolution of PG dynamics in vivo.

TL;DR: A strategy for exploitingtrehalose metabolic pathways to label glycolipids in mycobacteria with azide-modified trehalose (TreAz) analogues is described and unique labeling routes that can be harnessed for pathway-targeted investigation of theMycobacterial trehalome are identified.