J
John H. McCusker
Researcher at Duke University
Publications - 69
Citations - 8154
John H. McCusker is an academic researcher from Duke University. The author has contributed to research in topics: Saccharomyces cerevisiae & Gene. The author has an hindex of 39, co-authored 68 publications receiving 7764 citations. Previous affiliations of John H. McCusker include Brandeis University & Stanford University.
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Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae.
TL;DR: Three new dominant drug resistance cassettes have unique antibiotic resistance phenotypes and do not affect growth when inserted into the ho locus, which make the cassettes ideally suited for creating S. cerevisiae strains with multiple mutations within a single strain.
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Yeast microarrays for genome wide parallel genetic and gene expression analysis.
Deval A. Lashkari,Joseph L. DeRisi,John H. McCusker,Allen F. Namath,Cristl Gentile,Seung Y. Hwang,Patrick O. Brown,Ronald W. Davis +7 more
TL;DR: High-density DNA microarrays of yeast ORFs can monitor hybridization to ORFs for applications such as quantitative differential gene expression analysis and screening for sequence polymorphisms at the whole genome level.
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Dissecting the architecture of a quantitative trait locus in yeast
Lars M. Steinmetz,Himanshu Sinha,Daniel R. Richards,Jamie Spiegelman,Peter J. Oefner,John H. McCusker,Ronald W. Davis +6 more
TL;DR: This study combined genome-wide mapping and a new genetic technique named reciprocal-hemizygosity analysis to achieve the complete dissection of a quantitative trait locus (QTL) in Saccharomyces cerevisiae and uncovered a QTL architecture that was more complex than expected.
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Direct allelic variation scanning of the yeast genome.
Elizabeth A. Winzeler,Daniel R. Richards,Andrew R. Conway,Alan L. Goldstein,Sue Kalman,Michael McCullough,John H. McCusker,David A. Stevens,Lisa Wodicka,David J. Lockhart,Ronald W. Davis +10 more
TL;DR: It is demonstrated that allelic variation in any two isolates of a species can be scanned, mapped, and scored directly and efficiently without allele-specific polymerase chain reaction, without creating new strains or constructs, and without knowing the specific nature of the variation.
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The 100-genomes strains, an S. cerevisiae resource that illuminates its natural phenotypic and genotypic variation and emergence as an opportunistic pathogen
Pooja K Strope,Daniel A. Skelly,Stanislav G. Kozmin,Gayathri Mahadevan,Eric A. Stone,Paul M. Magwene,Fred S. Dietrich,John H. McCusker +7 more
TL;DR: Most phenotypic variation in S. cerevisiae strains is found to be quantitative and identified population, genotype, and phenotype associations, suggesting that copper resistance contributes to fitness in the human host.