J
John Hindley
Researcher at National University of Singapore
Publications - 12
Citations - 741
John Hindley is an academic researcher from National University of Singapore. The author has contributed to research in topics: Bacillus sphaericus & Peptide. The author has an hindex of 11, co-authored 12 publications receiving 725 citations.
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Cloning, sequencing, and expression of a gene encoding a 100-kilodalton mosquitocidal toxin from Bacillus sphaericus SSII-1.
TL;DR: The sequence of the SSII-1 toxin was compared with those of known toxins and was found to show regional homology to those of ADP-ribosyltransferase toxins.
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Binding of purified Bacillus sphaericus binary toxin and its deletion derivatives to Culex quinquefasciatus gut: elucidation of functional binding domains.
TL;DR: Highly larvicidal strains of Bacillus sphaericus produce a binary toxin which binds to sharply delineated regions of the gastric caecum and posterior midgut of susceptible larvae of the mosquito Culex quinquefasciatus and toxicity was strongly correlated with the subsequent internalization of the toxin, probably by endocytosis.
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Cytotoxicity and ADP-ribosylating activity of the mosquitocidal toxin from Bacillus sphaericus SSII-1: possible roles of the 27- and 70-kilodalton peptides.
TL;DR: Adomain in the N-terminal region of the Mtx protein is sufficient for ADP-ribosylation of C. quinquefasciatus cell protein, and a domain in the C-terminals is necessary for toxicity to cultured C.quinquefAsciatus cells; however, both regions are necessary foroxicity to mosquito larvae.
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Genetic determinants of host ranges of Bacillus sphaericus mosquito larvicidal toxins.
TL;DR: The patterns of larvicidal activity toward all three mosquito species and growth retardation in A. aegypti have been shown to be due to the 41.9-kDa protein, and mutant toxins expressed in Escherichia coli correlated with amino acids centered around position 100 of that protein.
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Expression of the mosquitocidal toxins of Bacillus sphaericus and Bacillus thuringiensis subsp. israelensis by recombinant Caulobacter crescentus, a vehicle for biological control of aquatic insect larvae
TL;DR: Caulobacter species are ubiquitous microorganisms residing in the upper regions of aquatic environments and therefore provide the potential for prolonged control by maintaining mosquitocidal toxins in larval feeding zones by cloning genes encoding the 51.4- and 41.9-kDa toxins from B. sphaericus 2297 and the transfer of these genes for expression in C. crescentus CB15 is described.