J
John William Chang
Researcher at University of the Pacific (United States)
Publications - 1
Citations - 120
John William Chang is an academic researcher from University of the Pacific (United States). The author has contributed to research in topics: Signal peptide & Peptide sequence. The author has an hindex of 1, co-authored 1 publications receiving 94 citations.
Papers
More filters
Journal ArticleDOI
The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris.
Geoff P. Lin-Cereghino,Carolyn M. Stark,Daniel Kim,Jennifer Chang,Nadia Shaheen,Hansel Poerwanto,Kimiko Agari,Pachai Moua,Lauren K. Low,Namphuong Tran,Amy Huang,Maria Nattestad,Kristin T. Oshiro,John William Chang,Archana G. Chavan,Jerry W. Tsai,Joan Lin-Cereghino +16 more
TL;DR: Though some mutations clearly dampened protein expression, deletion of amino acids 57-70, corresponding to the predicted 3rd alpha helix of α-mating factor secretion signal, increased secretion of reporter proteins horseradish peroxidase and lipase at least 50% in small-scale cultures, raising the possibility that the secretory efficiency of the leader can be further enhanced in the future.