J
Johnson Timothy
Researcher at Genzyme
Publications - 5
Citations - 398
Johnson Timothy is an academic researcher from Genzyme. The author has contributed to research in topics: Bioreactor & Chinese hamster ovary cell. The author has an hindex of 3, co-authored 5 publications receiving 363 citations.
Papers
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Journal ArticleDOI
Integrated continuous production of recombinant therapeutic proteins
Veena Warikoo,Rahul Godawat,Kevin Brower,Sujit Jain,Daniel Cummings,Elizabeth Simons,Johnson Timothy,Jason Walther,Marcella Yu,Benjamin Wright,Jean McLarty,Kenneth P. Karey,Chris Hwang,Weichang Zhou,Frank Riske,Konstantin Konstantinov +15 more
TL;DR: The first successful demonstration of the integration of a perfusion bioreactor and a four‐column periodic counter‐current chromatography system for the continuous capture of candidate protein therapeutics is reported, demonstrating the potential of integrated continuous bioprocessing as a universal platform for the manufacture of various kinds of therapeutic proteins.
Journal ArticleDOI
The effects of alternating tangential flow (ATF) residence time, hydrodynamic stress, and filtration flux on high-density perfusion cell culture.
TL;DR: Methods to estimate theoretical residence times of cells in the ATF system are developed and it is discovered that long residence times (above 75 s) correlate with decreased growth, metabolism, and productivity.
Patent
Methods of culturing a mammalian cell
Christopher Hwang,Johnson Timothy,Jason Walther,Cheng Cheng,Jonathan Wang,Neha Shah,Seul-A Bae +6 more
TL;DR: In this paper, the authors present methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.6g/L more, based on one or more factors selected from the group of pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.
Patent
Methods of culturing mammalian cell
Christopher Hwang,Johnson Timothy,Jason Walther,Cheng Cheng,Jonathan Wang,Neha Shah,Seul-A Bae +6 more
TL;DR: In this article, a method for Chinese hamster ovary cells (CHO) perfusion cultivation was described, under conditions sufficient for production of recombinant protein in a liquid medium containing poloxamer-188 in concentration 1.8 g/l or more 1.22 cl, 5 ex, 1 tbl, 15 dwg.
Patent
Methods for Improvidng Cell Viability In A Production Bioreactor
TL;DR: In this paper, the effect of a concentration of a sensitizer on cell viability in a production bioreactor has been investigated, and methods of improving cell viability and predicting cell viability have been proposed.