Frank Riske

TL;DR: The first successful demonstration of the integration of a perfusion bioreactor and a four‐column periodic counter‐current chromatography system for the continuous capture of candidate protein therapeutics is reported, demonstrating the potential of integrated continuous bioprocessing as a universal platform for the manufacture of various kinds of therapeutic proteins.

TL;DR: This paper presents a theoretical and practical approach for designing a periodic counter-current chromatography operation as a continuous capture purification step that is integrated with a perfusion cell culture process.

TL;DR: This paper examined chitosan as a flocculent of cells and cell particulates in NS0 culture harvest and the subsequent further clarification of this material by continuous flow centrifugation followed by depth and absolute filtration to improve material clarity and increasing material throughput.

TL;DR: This mini‐review is to provide an overview of the history of bioprocess AC, the current state of platform processes based on affinity capture steps, the maturing field of custom developed biobrocess affinity resins, and the advantages of affinity capture‐based downstream processing in comparison to other forms of chromatography.

TL;DR: A facile three column scalable production scheme, employing CB as the second column in the process was used to generate highly purified MAb from cell culture harvest derived from two media of very different compositions.