J
Joseph Sambrook
Researcher at University of Texas Southwestern Medical Center
Publications - 421
Citations - 265201
Joseph Sambrook is an academic researcher from University of Texas Southwestern Medical Center. The author has contributed to research in topics: DNA & RNA. The author has an hindex of 70, co-authored 413 publications receiving 262009 citations. Previous affiliations of Joseph Sambrook include University of Texas at Austin & Cold Spring Harbor Laboratory.
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Journal ArticleDOI
Analysis of RNA by Primer Extension
Michael R. Green,Joseph Sambrook +1 more
TL;DR: For mapping the 5' termini of mRNA molecules, primer extension is the method of choice and an enzyme cloned from the Moloney murine leukemia virus is used to carry out reverse transcription.
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Hybridization of Oligonucleotide Probes in Aqueous Solutions: Washing in Buffers Containing Quaternary Ammonium Salts.
Michael R. Green,Joseph Sambrook +1 more
TL;DR: In this protocol, hybridization is first performed in conventional aqueous solvents at a temperature well below the melting temperature, and the hybrids are then washed at higher stringency in buffers containing quaternary alkylammonium salts.
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Ecdysone as Regulator of Inducible Gene Expression in Mammalian Cells
Joseph Sambrook,David W. Russell +1 more
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Restriction endonuclease digestion of DNA in agarose plugs
Joseph Sambrook,David W. Russell +1 more
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Measurement of Radioactivity in Nucleic Acids.
Michael R. Green,Joseph Sambrook +1 more
TL;DR: Radioactive isotopes are used as tracers to monitor the progress of many reactions used to synthesize DNA and RNA and an effective method is differential precipitation of the nucleic acid products with trichloroacetic acid (TCA).