Kaemwich Jantama

TL;DR: Derivatives of Escherichia coli C were engineered to produce primarily succinate or malate in mineral salts media using simple fermentations (anaerobic stirred batch with pH control) without the addition of plasmids or foreign genes by combination of gene deletions and metabolic evolution.

TL;DR: Derivatives of Escherichia coli C were previously described for succinate production by combining the deletion of genes that disrupt fermentation pathways for alternative products with growth‐based selection for increased ATP production to create a strain devoid of foreign DNA.

TL;DR: Results of deleting individual transport genes confirmed that GalP served as the dominant glucose transporter in evolved strains and increased the pool of PEP available for redox balance by eliminating the need to produce additional PEP from pyruvate.

TL;DR: In these strains, reduced nicotinamide adenine dinucleotide oxidation during alanine biosynthesis is obligately linked to adenosine triphosphate production and cell growth, which provided a basis for metabolic evolution where selection for improvements in growth coselected for increased glycolytic flux andAlanine production.

TL;DR: In this paper, the fermentative metabolism of glucose was redirected to succinate as the primary product without mutating any genes encoding the native mixed-acid fermentation pathway or redox reactions.