K
Karl R. Koehler
Researcher at Indiana University
Publications - 43
Citations - 1939
Karl R. Koehler is an academic researcher from Indiana University. The author has contributed to research in topics: Inner ear & Induced pluripotent stem cell. The author has an hindex of 16, co-authored 34 publications receiving 1251 citations. Previous affiliations of Karl R. Koehler include University of Miami & University of Michigan.
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Generation of inner ear sensory epithelia from pluripotent stem cells in 3D culture
TL;DR: The stepwise differentiation of inner ear sensory epithelia from mouse embryonic stem cells (ESCs) in three-dimensional culture is reported, establishing a new in vitro model of innerEar differentiation that can be used to gain deeper insight into inner ear development and disorder.
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Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells
Karl R. Koehler,Jing Nie,Emma Longworth-Mills,Xiao Ping Liu,Jiyoon Lee,Jeffrey R. Holt,Eri Hashino +6 more
TL;DR: A method for differentiating human pluripotent stem cells to inner ear organoids that harbor functional hair cells and it is demonstrated that derived hair cells exhibit electrophysiological properties similar to those of native sensory hair cells.
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Hair-bearing human skin generated entirely from pluripotent stem cells
Jiyoon Lee,Jiyoon Lee,Cyrus C. Rabbani,Cyrus C. Rabbani,Hongyu Gao,Matthew R. Steinhart,Matthew R. Steinhart,Benjamin M. Woodruff,Zachary E. Pflum,Alexander Kim,Stefan Heller,Yunlong Liu,Taha Z. Shipchandler,Karl R. Koehler +13 more
TL;DR: It is demonstrated that nearly complete skin can self-assemble in vitro and be used to reconstitute skin in vivo, and that the skin organoids generated in vitro from human pluripotent stem cells are equivalent to the facial skin of human fetuses in the second trimester of development.
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Hair Follicle Development in Mouse Pluripotent Stem Cell-Derived Skin Organoids
TL;DR: It is shown that skin organoids spontaneously produce de novo hair follicles in a process that mimics normal embryonic hair folliculogenesis, useful for studying mechanisms of hair follicle induction, evaluating hair growth or inhibitory drugs, and modeling skin diseases.
Journal ArticleDOI
3D mouse embryonic stem cell culture for generating inner ear organoids
Karl R. Koehler,Eri Hashino +1 more
TL;DR: In this article, mouse embryonic stem cells are aggregated in 96-well plates in a medium containing extracellular matrix proteins to promote epithelialization, and the resulting inner ear hair cells and supporting cells are labeled as inner ear organoids.