Katharina Höhn

TL;DR: Heart-specific expression of constitutively active FoxO3 leads to reversible heart atrophy, which suggests a remarkable ability of the adult myocardium to respond to different regulatory cues.

TL;DR: Small features, so far only visible in transmission electron microscope (TEM) (e.g., the two leaflets of the membrane bi-layer, clathrin coats and cytoskeletal elements), can be resolved directly in the FIB/SEM in the 3D context of whole cells.

TL;DR: Three different approaches for three-dimensional imaging of electron microscopic samples are described, in which a sample is repeatedly milled with a focused ion beam (FIB) and each newly produced block face is imaged with the scanning electron microscope (SEM), and resolution is considerably improved when the secondary electron signal is used.

TL;DR: This combination of novel light transparent sapphire specimen carrier for high-pressure freezing and a freeze substitution protocol for better contrast of membranes allows for imaging membranes and other subcellular structures with unsurpassed quality.

TL;DR: This chapter describes optimized high-pressure freezing and freeze substitution protocols for STEM tomography in order to obtain high membrane contrast and adapted protocols for high- pressure freezing of cultivated cells from a physiological state.