A fast-Fourier-transform method of topography and interferometry is proposed. By computer processing of a noncontour type of fringe pattern, automatic discrimination is achieved between elevation and depression of the object or wave-front form, which has not been possible by the fringe-contour-generation techniques. The method has advantages over moire topography and conventional fringe-contour interferometry in both accuracy and sensitivity. Unlike fringe-scanning techniques, the method is easy to apply because it uses no moving components.

Fourier-transform method of fringe-pattern analysis for computer-based topography and interferometry

The iris, found in many animal species, is a biological tissue that can change the aperture (pupil) size to regulate light transmission into the eye in response to varying illumination conditions. The self-regulation of the eye lies behind its autofocusing ability and large dynamic range, rendering it the ultimate "imaging device" and a continuous source of inspiration in science. In optical imaging devices, adjustable apertures play a vital role as they control the light exposure, the depth of field, and optical aberrations of the systems. Tunable irises demonstrated to date require external control through mechanical actuation, and are not capable of autonomous action in response to changing light intensity without control circuitry. A self-regulating artificial iris would offer new opportunities for device automation and stabilization. Here, this paper reports the first iris-like, liquid crystal elastomer device that can perform automatic shape-adjustment by reacting to the incident light power density. Similar to natural iris, the device closes under increasing light intensity, and upon reaching the minimum pupil size, reduces the light transmission by a factor of seven. The light-responsive materials design, together with photoalignment-based control over the molecular orientation, provides a new approach to automatic, self-regulating optical systems based on soft smart materials.

Self-Regulating Iris Based on Light-Actuated Liquid Crystal Elastomer.

Confocal microscopes unlike their conventional counterparts have the ability to optically ‘section’ thick specimens. However the resolution and optical sectioning can be severely degraded by system or specimen-induced aberrations. The use of high aperture lenses further exacerbates the difficulties. We will describe an adaptive optics solution to this fundamental problem.

Adaptive optics in microscopy

The rapid development of artificial intelligence techniques and future advanced robot systems sparks emergent demand on the accurate perception and understanding of the external environments via visual sensing systems that can co-locate the self-adaptive detecting, processing, and memorizing of optical signals. In this contribution, a simple indium–tin oxide/Nb-doped SrTiO3 (ITO/Nb:SrTiO3) heterojunction artificial optoelectronic synapse is proposed and demonstrated. Through the light and electric field co-modulation of the Schottky barrier profile at the ITO/Nb:SrTiO3 interface, the oxide heterojunction device can respond to the entire visible light region in a neuromorphic manner, allowing synaptic paired-pulse facilitation, short/long-term memory, and “learning-experience” behavior for optical information manipulation. More importantly, the photoplasticity of the artificial synapse has been modulated by heterosynaptic means with a sub-1 V external voltage, not only enabling an optoelectronic analog of ...

An Oxide Schottky Junction Artificial Optoelectronic Synapse

The diverse vision systems found in nature can provide interesting design inspiration for imaging devices, ranging from optical subcomponents to digital cameras and visual prostheses, with more desirable optical characteristics compared to conventional imagers. The advantages of natural vision systems include high visual acuity, wide field of view, wavelength-free imaging, improved aberration correction and depth of field, and high motion sensitivity. Recent advances in soft materials, ultrathin electronics, and deformable optoelectronics have facilitated the realization of novel processes and device designs that mimic biological vision systems. This review highlights recent progress and continued efforts in the research and development of bioinspired artificial eyes. At first, the configuration of two representative eyes found in nature: a single-chambered eye and a compound eye, is explained. Then, advances in bioinspired optic components and image sensors are discussed in terms of materials, optical/mechanical designs, and integration schemes. Subsequently, novel visual prostheses as representative application examples of bioinspired artificial eyes are described.

Bioinspired Artificial Eyes: Optic Components, Digital Cameras, and Visual Prostheses

The shape of liquid interfaces can be precisely controlled using electrowetting, an actuation mechanism which has been widely used for tunable optofluidic micro-optical components such as lenses or irises. We have expanded the considerable flexibility inherent in electrowetting actuation to realize a variable optofluidic slit, a tunable and reconfigurable two-dimensional aperture with no mechanically moving parts. This optofluidic slit is formed by precisely controlled movement of the liquid interfaces of two highly opaque ink droplets. The 1.5 mm long slit aperture, with controllably variable discrete widths down to 45 µm, may be scanned across a length of 1.5 mm with switching times between adjacent slit positions of less than 120 ms. In addition, for a fixed slit aperture position, the width may be tuned to a minimum of 3 µm with high uniformity and linearity over the entire slit length. This compact, purely fluidic device offers an electrically controlled aperture tuning range not achievable with extant mechanical alternatives of a similar size. A variable optofluidic slit aperture that is based on electrowetting has been fabricated by researchers in Germany. Compact variable slit apertures are desired for use in miniaturized optical systems, but mechanical slits tend to be too bulky to be used in such systems. Now, Stefan Schuhladen and coworkers at the University of Freiburg have realized a tunable and reconfigurable two-dimensional optofluidic aperture that has no mechanically moving parts. It is formed by the precisely controlled movement of the liquid interfaces of two highly opaque ink droplets. The slit is 1.5 mm long and its width can be controlled down to 45 µm. It can be scanned across its length with a switching time of less than 120 ms between adjacent slit positions. This performance surpasses that of mechanical slits of the same size.

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Variable optofluidic slit aperture

We introduce a transmissive refractive adaptive optics system featuring a deformable transparent optofluidic wavefront modulator and a sensorless wavefront error estimation algorithm. The wavefront modulator consists of a cavity filled with an optical liquid which is sealed by a deformable elastic polymer membrane. Deformation of the membrane is achieved through electrostatic actuation using 25 transparent indium tin oxide electrodes buried in the cavity. Modulation of the two-dimensional phase distribution generated by the system is performed using open loop control both with and without active wavefront sensing. For the latter, a progressive modal decomposition algorithm is used to estimate and correct distortion in the point-spread function (PSF) of the wavefront arising due to the optical system and other sources of wavefront distortion. Using this control method, we experimentally demonstrate high-fidelity recreation of Zernike modes up to the fourth order, and blind (sensorless) PSF correction in a wide-field microscope.

Optofluidic adaptive optics

We discuss the implementation and performance of an adaptive optics (AO) system that uses two cascaded deformable phase plates (DPPs), which are transparent optofluidic phase modulators, mimicking the common woofer/tweeter-type astronomical AO systems. One of the DPPs has 25 electrodes forming a keystone pattern best suited for the correction of low-order and radially symmetric modes; the second device has 37 hexagonally packed electrodes better suited for high-order correction. We also present simulation results and experimental validation for a new open-loop control strategy enabling simultaneous control of both DPPs, which ensures optimum correction for both large-amplitude low-order, and complex combinations of low- and high-order aberrations. The resulting system can reproduce Zernike modes up to the sixth radial order with stroke and fidelity up to twice better than what is attainable with either of the DPPs individually. The performance of the new AO configuration is also verified in a custom-developed fluorescence microscope with sensorless aberration correction.

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Cascading optofluidic phase modulators for performance enhancement in refractive adaptive optics

We present a novel open-loop control method for an electrostatically actuated optofluidic refractive phase modulator, and demonstrate its performance for high-order aberration correction. Contrary to conventional electrostatic deformable mirrors, an optofluidic modulator is capable of bidirectional (push-pull) actuation through hydro-mechanical coupling. Control methods based on matrix pseudo-inversion, the common approach used for deformable mirrors, thus perform sub-optimally for such a device. Instead, we formulate the task of finding driving voltages for a given desired wavefront shape as an optimization problem with inequality constraints that can be solved using an interior-point method in real time. We show that this optimization problem is a convex one and that its solution represents a global minimum in residual wavefront error. We use the new method to control both the refractive phase modulator and a conventional electrostatic deformable mirror, and experimentally demonstrate improved correction fidelity for both.

Optimization-based real-time open-loop control of an optofluidic refractive phase modulator.

Sample-induced optical aberrations in microscopy are, in general, field dependent, limiting their correction via pupil adaptive optics (AO) to the center of the available field-of-view (FoV). This is a major hindrance, particularly for deep tissue imaging, where AO has a significant impact. We present a new wide-field AO microscopy scheme, in which the deformable element is located at the pupil plane of the objective. To maintain high-quality correction across its entirety, the FoV is partitioned into small segments, and a separate aberration estimation is performed for each via a modal-decomposition-based indirect wavefront sensing algorithm. A final full-field image is synthesized by stitching of the partitions corrected consecutively and independently via their respective measured aberrations. The performance and limitations of the method are experimentally explored on synthetic samples imaged via a custom-developed AO fluorescence microscope featuring an optofluidic refractive wavefront modulator.

Kaustubh Banerjee

Papers

Variable optofluidic slit aperture

Optofluidic adaptive optics

Cascading optofluidic phase modulators for performance enhancement in refractive adaptive optics

Optimization-based real-time open-loop control of an optofluidic refractive phase modulator.

Extended field-of-view adaptive optics in microscopy via numerical field segmentation