K
Kazuchika Miyoshi
Researcher at Kagoshima University
Publications - 48
Citations - 689
Kazuchika Miyoshi is an academic researcher from Kagoshima University. The author has contributed to research in topics: Somatic cell nuclear transfer & Blastocyst. The author has an hindex of 15, co-authored 45 publications receiving 625 citations.
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Journal ArticleDOI
Valproic acid enhances in vitro development and Oct-3/4 expression of miniature pig somatic cell nuclear transfer embryos
Kazuchika Miyoshi,Hironori Mori,Yamato Mizobe,Eri Akasaka,Akio Ozawa,Mitsutoshi Yoshida,Masahiro Sato +6 more
TL;DR: In this paper, the authors examined the effects of valproic acid (VPA), a histone deacetylase inhibitor, on in vitro development of miniature pig somatic cell nuclear transfer (SCNT) embryos and on expression of a mouse Oct-3/4 promoter-driven enhanced green fluorescent protein (EGFP) gene.
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Enhancement of Cytoplasmic Maturation of In Vitro-Matured Pig Oocytes by Mechanical Vibration
TL;DR: It was shown that in vitro maturation of oocytes with mechanical vibration can be applied to efficient production of blastocysts derived from miniature pig somatic cell nuclear transfer (SCNT) embryos.
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Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations.
Masahiro Sato,Miyu Koriyama,Satoshi Watanabe,Masato Ohtsuka,Takayuki Sakurai,Emi Inada,Issei Saitoh,Shingo Nakamura,Kazuchika Miyoshi +8 more
TL;DR: It is suggested that zygote microinjection of mRNAs might lead to the production of piglets with cells harboring various mutation types, and a correlation between EGFP expression and mutation induction is suggested.
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The combinational use of CRISPR/Cas9-based gene editing and targeted toxin technology enables efficient biallelic knockout of the α-1,3-galactosyltransferase gene in porcine embryonic fibroblasts.
Masahiro Sato,Kazuchika Miyoshi,Yozo Nagao,Yohei Nishi,Masato Ohtsuka,Shingo Nakamura,Takayuki Sakurai,Satoshi Watanabe +7 more
TL;DR: Using the CRISPR/Cas9 system, it is aimed to destroy the function of the porcine α‐1,3‐galactosyltransferase (α‐GalT) gene (GGTA1) whose product is responsible for the synthesis of the α‐Gal epitope, a causative agent for hyperacute rejection upon pig‐to‐human xenotransplantation.
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Utility of ultrasound stimulation for activation of pig oocytes matured in vitro.
TL;DR: It is shown that ultrasound stimulation can induce the nuclear activation and parthenogenetic development of pig oocytes matured in vitro under various conditions.