Kohei Nishimura

TL;DR: The auxin-inducible degron (AID) system allowed rapid and reversible degradation of target proteins in response to auxin and enabled us to generate efficient conditional mutants of essential proteins in yeast as well as cell lines derived from chicken, mouse, hamster, monkey and human cells, thus offering a powerful tool to control protein expression and study protein function.

TL;DR: It is demonstrated that the Elg1 replication factor C-like complex (Elg1-RLC) functions in unloading of both unmodified and SUMOylated PCNA during DNA replication, while the genome instability of an elg1Δ mutant suggests timely PCNA unloading is critical for chromosome maintenance.

TL;DR: It is shown that the replicative helicase-related Mcm family of proteins, Mcm8 and Mcm9, forms a complex required for HR repair induced by ICLs, and is required forHR that promotes sister chromatid exchanges, probably as a hexameric ATPase/helicase.

TL;DR: It is proposed that the off-AID system is the powerful method for in vivo protein-depletion in fission yeast.

TL;DR: This protocol describes a procedure to generate AID mutants of budding yeast via a simple transformation using PCR‐amplified DNA and methods to confirm the depletion of proteins of interest that are required for proliferation by serial‐dilution and liquid‐culture assays.