Krzysztof Kaczmarski

TL;DR: The discussion of the contribution to the HETP of the mass-transfer resistances shows that shell particles exhibit much lower plate heights for large molecular size compounds than do fully porous particles, this advantage increasing with decreasing thickness of the shell.

TL;DR: Using columns packed with Halo, a new type of porous silica shell particles, the gain achieved with this principle for peptides of moderate molecular weights and for small proteins is assessed.

TL;DR: The experimental results of a previous study of the mass transfer kinetics of bovine serum albumin in ion-exchange chromatography were reevaluated using the general rate model of chromatography, showing that the use of an oversimplified model for the analysis of chromatographic data can lead to erroneous interpretations of the experimental data and to misunderstandings of the fundamentals of the processes involved.

TL;DR: The results derived from the three sets of results show consistently that annealing reduces the energetic heterogeneity of the polymer surface for both LPA and DPA.

TL;DR: A model combining the heat and mass balance of the column, the equations of flow through porous media, and a linear isotherm model of the analyte is solved and validated for conventional columns operated under moderate pressures and results prove that this model accounts well for all the experimental results.