Showing papers in "Journal of Chromatography A in 2002"

TL;DR: The current state of development in the area of membrane chromatographic separation of proteins is reviewed and the technical challenges facing membrane chromatography are highlighted and the scope for future work is discussed.

TL;DR: Characteristics of the method are high recoveries of analyzed phytohormones and their sufficient purity for quantification by HPLC-ELISA (RIA) orHPLC-MS.

TL;DR: Monolithic silica columns prepared from tetraalkoxysilane by a sol-gel method showed high efficiency and high permeability on the basis of the small-sized silica skeletons, large-sized through-pores, and resultingThrough-pore size/skeleton size ratios much larger than those found in a particle-packed column.

TL;DR: The analysis of spiked samples has shown that there is good agreement between the real mass of compound added to the wine and that determined by analysis, and detection limits are below the odor detection threshold of the compounds.

TL;DR: The chemical analysis and quality control of Ginkgo leaves and extracts is reviewed in this paper, where the terpene trilactones are extracted with aqueous acetone and supercritical fluid extraction.

TL;DR: A survey of the literature dealing with practical applications and recent meetings shows that preparative liquid chromatography is becoming a well established separation and purification method in the pharmaceutical industry as discussed by the authors.

TL;DR: A fast and cost effective method was developed to extract and quantify residues of veterinary antimicrobial agents (antibiotics) in animal manure by liquid-liquid extraction and liquid chromatography-mass spectrometry, which revealed total sulfonamide concentrations of up to 20 mg/kg liquid manure.

TL;DR: This review updates knowledge about pressurised liquid extraction (PLE) and subcritical water extraction (SWE), two sample preparation techniques which are increasingly used for the extraction of moderately and non-volatile organic pollutants from a variety of solid and semi-solid environmental matrices.

TL;DR: A monolithic stationary phase is the continuous unitary porous structure prepared by in situ polymerization or consolidation inside the column tubing and the surface is functionalized to convert it into a sorbent with the desired chromatographic binding properties.

TL;DR: An overview of the latest developments in the key device subject areas and the basic interdisciplinary technologies is provided, along with applications for this novel "synergized" technology in high-throughput separations of biologically important molecules.

TL;DR: Packed microcolumns using self-assembled particles (SAPs) as frits that are smaller than the size of the outlet are developed, with a five to one ratio of outlet size to particle diameter appearing to be the upper maximum.

TL;DR: Although a column with smaller domain size generally resulted in higher separation impedance and the lower total performance, the monolithic silica columns showed performance beyond the limit of conventional particle-packed columns under pressure-driven conditions.

TL;DR: Superheated water extraction is cleaner, faster and cheaper than the conventional extraction methods and gives comparable results to Soxhlet extraction but more rapidly and without the use of significant volumes of organic solvents.

TL;DR: The HPLC method was successfully used to measure a variety of organic acids and phenolic compounds in juices and beverages and could also be used to evaluate the authenticity, spoilage or micronutrient contents of juices.

TL;DR: An analysis of system constants for gas Chromatography, liquid chromatography, supercritical fluid chromatography and micellar electrokinetic chromatography as a function of different experimental variables is performed as a step towards gaining a theoretical understanding of selectivity optimization for method development.

TL;DR: From tens to several thousand-fold improvements in detector response for many neutral and charged analytes have been achieved with this technique, suggesting sweeping as a general approach to on-line preconcentration in CE.

TL;DR: High performance liquid chromatography on reversed-phase columns remains the best technique for saponin determination and is the most-widely used method for this group of compounds.

TL;DR: Results indicate that microbial cells can be handled in a chromatographic mode using supermacroporous continuous columns, which are easy to manufacture from cheap and readily available starting materials, which make the columns suitable for single-time use.

TL;DR: A liquid chromatography-electrospray ionization tandem mass spectrometry method was developed and validated for simultaneous analysis of nine acidic pharmaceutical drugs in sewage treatment plant (STP) effluents in which bezafibrate, diclofenac, fenoprofen, gemfibrozil, ibuprofen and naproxen were detected.

TL;DR: Nine samples of lavender essential oil were analysed by GC-MS using low-polarity and polar capillary columns and linear retention indices (LRI) were calculated for each component detected, finding more components could be found within the two-dimensional separation space.

TL;DR: GC-MS and GC-MS-MS were used to determine 4-NP, 4-tert.-octylphenol (4-t-OP), bisphenol A, estradiol-17beta, estriol estrone, testosterone, 17alpha-ethynylestradioL cholesterol,Coprostan-3-ol, coprostan and coproStan- 3-one in both surface water and wastewater after derivatization with N,O-

TL;DR: A time warping algorithm for alignment of LC-MS data in the chromatographic direction has been examined and with moderate time shifts present in the data, pre-processing with this algorithm yields approximately trilinear data for which reasonable models can be made.

TL;DR: An overview of mass spectrometric methods used for the determination of endocrine disrupting compounds (EDCs) in environmental samples is presented and various aspects of current LC-MS and GC-MS methodology, including sample preparation, are discussed.

TL;DR: All significant contributions to column selectivity have been identified (and can be measured) for individual alkyl-silica columns which do not have an embedded polar group, suggesting that columns of the latter kind can be quantitatively characterized in terms of selectivity for use in the separation of any sample.

TL;DR: The review covers most of the methods described for the determination of quinolone residues in edible animal products and a summary of the most relevant information about the analytical procedures has been included.

TL;DR: The monolithic silica columns showed performance beyond the limit of conventional particle-packed columns in HPLC under favorable conditions, and for solutes with large retention factors due to the slow mobile-phase mass transfer in the large through-pores.

TL;DR: A GC-sniffing technique was used to correlate the single odor impression of the identified SPME headspace volatiles of the three investigated pepper samples with the following results: themain compounds such as beta-caryophyllene, germacrene D, limonene, beta-pinene, alpha-phellandrene and alpha-humulene, as well as minor constituents.

TL;DR: It was demonstrated that GC X GC improves the separation dramatically and all 58 pesticides of interest could be identified using their full-scan mass spectra, which was not possible when using ID-GC-TOF MS.

TL;DR: In this article, the potential and current limitations of comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry (GC×GC-TOF-MS) for the analysis of very complex samples were studied with the separation of cigarette smoke as an example.

TL;DR: The work described in this paper tests the efficacy of a global isotope labeling (global internal standard technology, GIST) strategy for quantification in proteomics and found MS-MS was found to be uniquely suited for isobaric peptide quantification.