L
Lakey
Researcher at University of Alberta
Publications - 17
Citations - 835
Lakey is an academic researcher from University of Alberta. The author has contributed to research in topics: Islet & Cryopreservation. The author has an hindex of 11, co-authored 17 publications receiving 815 citations.
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Journal ArticleDOI
Variables in organ donors that affect the recovery of human islets of Langerhans.
Lakey,Garth L. Warnock,Ray V. Rajotte,Suarez-Alamazor Me,Ziliang Ao,A. M. J. Shapiro,N. M. Kneteman +6 more
TL;DR: In an attempt to reduce the variability in the yields of human islet isolations and to identify donor factors that were potentially deleterious, retrospectively reviewed 153 human islets isolations in the center over a 3-year period suggests an improved approach to the prediction of successful islet isolation from human pancreases.
Journal ArticleDOI
Intraductal collagenase delivery into the human pancreas using syringe loading or controlled perfusion.
Lakey,Garth L. Warnock,A. M. J. Shapiro,Gregory S. Korbutt,Ziliang Ao,Norman M. Kneteman,Ray V. Rajotte +6 more
TL;DR: Controlled perfusion via the pancreatic duct allows the effective delivery of the enzyme achieving maximal distension to all regions of the pancreas leading to an increased recovery of the islets with no detrimental effect on subsequent in vitro islet function.
Journal ArticleDOI
Portal vein thrombosis after transplantation of partially purified pancreatic islets in a combined human liver/islet allograft.
A. M. J. Shapiro,Lakey,Ray V. Rajotte,Garth L. Warnock,Friedlich Ms,Laurence D. Jewell,Norman M. Kneteman +6 more
Journal ArticleDOI
Serine-protease inhibition during islet isolation increases islet yield from human pancreases with prolonged ischemia.
Lakey,Lisa M. H. Helms,Tatsuya Kin,Gregory S. Korbutt,Ray V. Rajotte,A. M. J. Shapiro,Garth L. Warnock +6 more
TL;DR: Inhibition of intrinsic protease activity within pancreases after prolonged cold storage improves isolation of viable islets.
Journal ArticleDOI
Heterogenous expression of nestin in human pancreatic tissue precludes its use as an islet precursor marker.
Cale Street,Lakey,K Seeberger,Lisa M. H. Helms,Ray V. Rajotte,A. M. J. Shapiro,Gregory S. Korbutt +6 more
TL;DR: A diffuse and variable expression of nestin in human pancreas is shown that may be due to a number of different processes, including post-mortem tissue remodeling and cellular differentiation.