Integrins: versatility, modulation, and signaling in cell adhesion.

Traffic signals for lymphocyte recirculation and leukocyte emigration: The multistep paradigm

The adhesive interactions of cells with other cells and with the extracellular matrix are crucial to all developmental processes, but have a central role in the functions of the immune system throughout life Three families of cell-surface molecules regulate the migration of lymphocytes and the interactions of activated cells during immune responses

Adhesion receptors of the immune system.

Scaffolds in tissue engineering bone and cartilage.

Hydrogels for tissue engineering.

Decreased deform ability of erythrocytes and increased intracellular calcium in patients with chronic renal failure

As calcium has increasingly been the object of study in erythrocyte physiology, we reviewed the current methodologies for determination of calcium by atomic absorption spectrometry. The published normal values for erythrocyte calcium vary from 5 to 500 mumol/liter of packed cells. A method based on Harrison and Long's determination of calcium is presented and shows normal red cell calcium concentration to be 0.0149 +/- 0.0023 mumol/ml of packed red cells. The influence of temperature and type of crucible used in ashing red cells is assessed. The method of additions is employed to corroborate our results.

Problems in measurement of erythrocyte calcium

Mechanical trauma effects on leukocytes.

Whether platelet microtubules are involved in clot retraction/contraction has been controversial. To address this question we have simultaneously measured two clotting parameters, clot structural rigidity and isometric contractile force, using a rheological technique. For recalcified PRP clots these two parameters began rising together at about 15 min after CaCl2 addition. In the concentration range affecting microtubule organization in platelets, colchicine, vinca alkaloids and taxol demonstrated insignificant effects on both clotting parameters of a recalcified PRP clot. For PRP clots induced by adding small amounts of exogenous thrombin, the kinetic curves of clot rigidity were biphasic and without a lag time. The first phase corresponded to a platelet-independent network forming process, while the second phase corresponded to a platelet-dependent process. These PRP clots began generating contractile force at the onset of the second phase. For both rigidity and force parameters, only the second phase of clotting kinetics was retarded by microtubule affecting reagents. When PRP samples were clotted by adding a mixture of CaCl2 and thrombin, the second phase clotting was accelerated and became superimposed on the first phase. The inhibitory effects of microtubule affecting reagents became less pronounced. Thrombin clotting of a two-component system (washed platelets/purified fibrinogen) was also biphasic, with the second phase being microtubule-dependent. In conclusion, platelet microtubules are important in PRP clotted with low concentrations of thrombin, during which fibrin network formation precedes platelet-fibrin interactions. On the other hand they are unimportant if a PRP clot is induced by recalcification, during which the fibrin network is constructed in the presence of platelet-fibrin interactions.(ABSTRACT TRUNCATED AT 250 WORDS)

Platelet microtubules in clot structure formation and contractile force generation: investigation of a controversy.

The direct interaction between B-cells, both normal and neoplastic, and marrow stromal cells (MSC) or MSC-secreted extracellular matrix proteins is believed to play a decisive role in lymphopoiesis as well as in the sustained proliferation and homing of lymphoid malignancies. Although information has been accruing on possible obligatory cell-cell and cell-extracellular matrix adhesion molecules, our current state of knowledge regarding B-cell interaction with the marrow microenvironment is rather limited. We discuss in this review what is currently known regarding adhesion molecules involved in the apposition between B-cells and MSC. In addition, we describe how the cellular interactions are modulated by cytokines present in the marrow microenvironment. Further, we present possible signal transduction pathways activated by B-cell/MSC apposition and attempt to integrate in vitro data with in vivo observations. We close this review by providing implications for novel clinical and therapeutic applications ba...

Larry V. McIntire

Papers

Decreased deform ability of erythrocytes and increased intracellular calcium in patients with chronic renal failure

Problems in measurement of erythrocyte calcium

Mechanical trauma effects on leukocytes.

Platelet microtubules in clot structure formation and contractile force generation: investigation of a controversy.

Cellular interactions among marrow stromal and normal/neoplastic pre-B- and B-lymphoblastic cells