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Laurent Jannière

Researcher at Institut national de la recherche agronomique

Publications -  37
Citations -  1702

Laurent Jannière is an academic researcher from Institut national de la recherche agronomique. The author has contributed to research in topics: DNA replication & Plasmid. The author has an hindex of 24, co-authored 34 publications receiving 1630 citations. Previous affiliations of Laurent Jannière include Université Paris-Saclay & Epigenomics AG.

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Structurally stable Bacillus subtilis cloning vectors

TL;DR: It is suggested that the high structural stability of large plasmid is a consequence of their mode of replication and that plasmids which do not replicate as rolling circles should be used whenever it is necessary to clone and maintain long DNA segments in any organism.
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Two essential DNA polymerases at the bacterial replication fork.

TL;DR: Bacillus subtilis genome analysis shows that dnaEBS is essential for cell viability and for the elongation step of DNA replication, as is polC, and concludes that there are two different essential DNA polymerases at the replication fork of B. subtil is, as was previously observed in eukaryotes.
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A fourth class of theta-replicating plasmids: the pAM beta 1 family from gram-positive bacteria

TL;DR: In this article, it was shown that pAM beta 1 is dependent on a plasmid-encoded replication protein (Rep) but not on a DNA structure typical for origins of most Rep-dependent plasmids and is initiated by DNA polymerase I (PolI).
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Unidirectional theta replication of the structurally stable Enterococcus faecalis plasmid pAM beta 1.

TL;DR: This work confirms the use of termination signals to localize origins, suggests that termination in B. subtilis occurs by a mechanism similar to that of Escherichia coli and establishes that in addition to rolling circle replicating plasmids, Gram positive bacteria harbour plasmid which replicate by a theta mechanism.
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Primosome assembly site in Bacillus subtilis.

TL;DR: For the first time, a primosome is described in a Gram‐positive bacterium and involvement of the B.subtilis proteins DnaB, DnaD and DnaI, which do not have any known analogue in Escherichia coli, raises the possibility that primosomes assembly and/or function in B. Subtilis differs from that in E.coli.