Lawrence T. Feldman

TL;DR: In initial attempts to define the molecular events responsible for the latent state of herpes simplex virus, in situ hybridization was utilized to search for virally encoded RNA transcripts in latently infected sensory neurons, finding only RNA transcripts hybridizing to the ICP-0 probe were detected.

TL;DR: It is reported here that LAT is actually a uniquely stable intron, and effectively inhibits transactivation of gene expression by infected-cell polypeptide 0 in transient transfection assays.

TL;DR: A genetically engineered herpes simplex virus variant was constructed for use as a stable gene vector for neurons and established latent infections and stably expressed beta-galactosidase in primary sensory neurons.

TL;DR: It is concluded that productive cycle viral genes are abundantly expressed in rare neurons of latently infected murine TG and that these events are promptly recognized by an active local immune response.

TL;DR: Data suggest that the herpes simplex virus type 1 latency-associated transcript (LAT) may be processed from a larger transcription unit which begins distal to the TATA box 700 base pairs upstream of the LAT and extends to a polyadenylation signal almost 5 kilobases downstream of the 3' end ofThe LAT.