L
Leah Tolosa
Researcher at University of Maryland, Baltimore County
Publications - 79
Citations - 1641
Leah Tolosa is an academic researcher from University of Maryland, Baltimore County. The author has contributed to research in topics: Glucose binding & Analyte. The author has an hindex of 20, co-authored 79 publications receiving 1515 citations. Previous affiliations of Leah Tolosa include University of Maryland, Baltimore & University of Connecticut.
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Journal ArticleDOI
Glucose Sensor for Low-Cost Lifetime-Based Sensing Using a Genetically Engineered Protein☆☆☆
Leah Tolosa,Ignacy Gryczynski,Lisa Eichhorn,Jonathan D. Dattelbaum,Felix N. Castellano,Govind Rao,Joseph R. Lakowicz +6 more
TL;DR: A glucose sensor based on a mutant glucose/galactose binding protein (GGBP) and phase-modulation fluorometry and results suggest an approach to glucose sensing with simple devices are suggested.
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Optical assay for glucose based on the luminescnence decay time of the long wavelength dye Cy5
TL;DR: An optical assay for glucose is described based on the luminescence decay time of a long wavelength dye (Cy5) which can be excited with currently available red laser diodes which can result in practical real world assays for glucose.
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Noninvasive measurement of dissolved oxygen in shake flasks.
TL;DR: This is the first time that in situ oxygen limitation is reported in shake flasks, and the sensitivity and accuracy of this device is maximal up to 60% DO, within the range that is critical to cell culture applications.
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Lifetime-Based Sensing of Glucose Using Energy Transfer with a Long Lifetime Donor
TL;DR: An optical assay for glucose based on the luminescence decay time of a long lifetime metal-ligand complex which allows phase-modulation decay time measurements using an amplitude-modulated bluelight-emitting diode as the light source.
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Dual-labeled glucose binding protein for ratiometric measurements of glucose.
Xudong Ge,Leah Tolosa,Govind Rao +2 more
TL;DR: A ratiometric glucose sensor capable of measuring micromolar levels of glucose based on an E. coli glucose binding protein labeled with two fluorophores and the presence of the long-lived ruthenium metal ligand complex allows for low-cost modulation-based sensing.