The glycoprotein hormone erythropoietin regulates the level of oxygen in the blood by modulating the number of circulating erythrocytes, and is produced in the kidney or liver of adult and the liver of fetal or neonatal mammals. Neither the precise cell types that produce erythropoietin nor the mechanisms by which the same or different cells measure the circulating oxygen concentration and consequently regulate erythropoietin production are known. Cells responsive to erythropoietin have been identified in the adult bone marrow, fetal liver or adult spleen. In cultures of erythropoietic progenitors, erythropoietin stimulates proliferation and differentiation to more mature red blood cells. Detailed molecular studies have been hampered, however, by the impurity and heterogeneity of target cell populations and the difficulty of obtaining significant quantities of the purified hormone. Highly purified erythropoietin may be useful in the treatment of various forms of anaemia, particularly in chronic renal failure. Here we describe the cloning of the human erythropoietin gene and the expression of an erythropoietin cDNA clone in a transient mammalian expression system to yield a secreted product with biological activity.

Isolation and characterization of genomic and cDNA clones of human erythropoietin

The role of surface carbohydrates in the hepatic recognition and transport of circulating glycoproteins.

The Biochemistry of glycoproteins and proteoglycans

Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation

The isolation and properties of a rabbit liver binding protein specific for asialoglycoproteins.

Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins.

http://www.jbc.org/content/247/14/4633.full.pdf

Lee Van Lenten

Papers

Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins.

The binding of desialylated glycoproteins by plasma membranes of rat liver. Development of a quantitative inhibition assay.

[15] Tritium-labeling of glycoproteins that contain sialic acid