Lihi Shaulov

TL;DR: High-resolution crystal structures of sequence-specific complexes between the core domain of human p53 and different DNA half-sites are presented and establish a structural framework for understanding the mechanisms of specificity, affinity, and cooperativity of DNA binding by p53.

TL;DR: The genome of this phage encodes an entirely unique set of structural proteins not found in any currently known phage, indicating that it uses lineage-specific genes for virion morphogenesis and represents a previously unknown lineage of myoviruses.

TL;DR: It is shown that the initial step of chromatin seeding is negatively regulated by importin beta, and suggested that initiation sites for NPC assembly contain single copies of Chromatin-bound ELYS/Nup107-160 and that the lateral oligomerization of these subunits depends on the recruitment of membrane components.

TL;DR: It is demonstrated that the CWWG motif, found in the center of each consensus p53 half-site, is a key player in p53/DNA interactions and binds to p53 consensus sites containing CATG with relatively low cooperativity, as both dimers and tetramers, and with even lower cooperativity to such sites containing spacer sequences.

TL;DR: It is shown that a soluble internal fragment of the membrane nucleoporin POM121 has a dominant-negative effect on both modes of assembly in a cell-free reconstitution system and links nuclear membrane formation and expansion to nuclear pore biogenesis.