Itai Yanai

TL;DR: It is shown that CEL-Seq gives more reproducible, linear, and sensitive results than a PCR-based amplification method, and will be useful for transcriptomic analyses of complex tissues containing populations of diverse cell types.

TL;DR: A droplet-based, single-cell RNA-seq method is implemented to determine the transcriptomes of over 12,000 individual pancreatic cells from four human donors and two mouse strains and provides a resource for the discovery of novel cell type-specific transcription factors, signaling receptors, and medically relevant genes.

TL;DR: The analyses provide a novel route to infer expression profiles for presumed ancestral nodes in the tissue dendrogram, whereby de novo enhancement and diminution of gene expression go hand in hand, and highlight the importance of gene suppression events.

TL;DR: Improvements in economics, resolution, and ease of use make CEL-Sequ2 uniquely suited to single-cell RNA-Seq analysis in terms of economics,resolution, and easing of use.

TL;DR: The results of this analysis suggest the following genome expansion history: first, the generation of a "tetrapod-specific" Class II OR cluster on chromosome 11 by local duplication, then a single-step duplication of this cluster to chromosome 1, and finally an avalanche of duplication events out of chromosome 1 to most other chromosomes.