Linjuan Huang

TL;DR: A novel one‐pot procedure for the nonreductive release of O‐linked glycans from glycoproteins and the simultaneous derivatization of released glycans with 1‐phenyl‐3‐methyl‐5‐pyrazolone (PMP) is described, which has shown methodological significance in O‐glycan analysis.

TL;DR: A water-soluble polysaccharide with a protein content of 3.75%, designated as LbGp1, was isolated from the fruit of Lycium barbarum L. barbarum and structure was characterized based on monosaccharide composition, partial hydrolysis, methylation analysis and electrospray ionization mass spectrometer (ESI-MS).

TL;DR: A complex polysaccharide, termed LRP4-A, was isolated from the fruit of Lycium ruthenicum Murr and its structure was characterized using a series of analytical techniques, including methylation analysis, partial acid hydrolysis, IR, NMR, and ESI-MS.

TL;DR: A simplified quantitative glycomics strategy, which features nonreductive isotopic labeling of reducing glycans with either nondeuterated or deuterated Girard's reagent P (GP) without salts introduced and simplified mass spectrometric profiles of d0- and d5-GP derivatives of neutral glycans as molecular ions without complex metal ion adducts, allowing rapid and sensitive quantitative comparison between different glycan samples.

TL;DR: Girard's reagent T is employed for on-target derivatization (GTOD) of reducing glycan under mild acid condition to form stable hydrazones at room temperature, allowing rapid and sensitive identification of neutral and sialylated glycans in positive-ion mode as only permanently positive charged molecular ions without multiple ion adducts by MALDI-TOF-MS.