Lisa Goers

TL;DR: An overview of existing experimental and theoretical co-culture set-ups in synthetic biology and adjacent fields is given here, and challenges and opportunities involved in such experiments are discussed.

TL;DR: A distinct mechanism of pathogen-sensing mediated by an immune sensor protein called NLRP1B, a member of the nucleotide-binding domain leucine-rich repeat (NLRP) protein superfamily, is described, which finds that N-terminal cleavage of NL RP1B by LF protease results in destabilization of NLRP 1B and its degradation by the proteasome.

TL;DR: It is demonstrated that intestinal epithelial cell (IEC)-specific NAIP–NLRC4 activity is sufficient to protect mice from shigellosis, and the results suggest that the lack of an inflammasome response in IECs may help explain the susceptibility of humans to Shigella.

TL;DR: A whole‐cell Escherichia coli lactate biosensor based on the lldPRD operon is developed and successfully used to monitor lactate concentration in mammalian cell cultures and serves as a blueprint for how to capitalize on the wealth of genetic operons for metabolite sensing available in nature for the development of other whole-cell biosensors.

TL;DR: In this article , the authors summarize the latest progress in rationally engineered variants of Escherichia coli Nissle 1917 (EcN) for the treatment of infectious diseases, metabolic disorders, and inflammatory bowel diseases.