Showing papers by "Lorena Wilson published in 2015"
TL;DR: The catalysis of asymmetric esterification of glycerol with benzoic acid to obtain R -α-MBG by CALB immobilized in heterofunctional silica is highly selective and, to the authors' knowledge, is the most selective reported to date.
48 citations
TL;DR: The highest lactulose palmitate yields were obtained at 47 °C in acetone, for all biocatalysts, while the best lactulOSE:palmitic acid molar ratio differed according to the immobilization methodology, being 1:1 for AsL-OGS bioc atalyst and 1:3 for the others.
Abstract: Sugar esters are considered as surfactants due to its amphiphilic balance that can lower the surface tension in oil/water mixtures. Enzymatic syntheses of these compounds are interesting both from economic and environmental considerations. A study was carried out to evaluate the effect of four solvents, temperature, substrate molar ratio, biocatalyst source, and immobilization methodology on the yield and specific productivity of lactulose palmitate monoester synthesis. Lipases from Pseudomonas stutzeri (PsL) and Alcaligenes sp. (AsL), immobilized in porous silica functionalized with octyl groups (adsorption immobilization, OS) and with glyoxyl-octyl groups (both adsorption and covalent immobilization, OGS), were used. The highest lactulose palmitate yields were obtained at 47 °C in acetone, for all biocatalysts, while the best lactulose:palmitic acid molar ratio differed according to the immobilization methodology, being 1:1 for AsL-OGS biocatalyst (20.7 ± 3%) and 1:3 for the others (30-50%).
35 citations
TL;DR: The high purity S -methyl-3-phenyl glutarate produced in sequential batch reactor operation shows that the biocatalyst can be reused at least twice without losing stereoselectivity, favoring a reduction in the process cost.
34 citations
TL;DR: Combi-CLEAs of glycosidases are technologically relevant robust biocatalysts for their application as aroma enhancers in winemaking and are more stable than the soluble enzymes.
24 citations