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Showing papers in "Process Biochemistry in 2015"


Journal ArticleDOI
TL;DR: In this paper, a critical review focusing on biofilm reactors for anaerobic treatment of dairy wastewaters of varying strengths is provided, where the effects of organic loading rate and hydraulic retention time on the performance of bioreactors are discussed and operational problems are summarized.

205 citations


Journal ArticleDOI
TL;DR: A brief overview of the role of biocatalysts in the synthesis of various cosmetic esters can be found in this article, where the application of novel green techniques such as ultrasonication and microwave irradiation to address the drawbacks of the conventional enzymatic technique.

155 citations


Journal ArticleDOI
TL;DR: This review considers different solvent systems used in lipase-catalyzed reactions and some of the enzymatic properties required for function and gives lipases many potential applications in aqueous and non-aqueous biocatalysis.

150 citations


Journal ArticleDOI
TL;DR: In this paper, the authors provide an overview of enzymatic profiles for several ascomycetes covering phylogenetically distinct genera and species, and provide guidelines for directly comparing cellulolytic enzyme production in SSF with SmF to advance future research of enzyme production.

122 citations


Journal ArticleDOI
Dan Li1, Zuojia Liu1, Ye Yuan1, Yawen Liu1, Fenglan Niu1 
TL;DR: GA-AgNPs could be a new potential candidate for use in biological and pharmaceutical applications for fighting infections caused by microorganisms, according to a simple and green method.

93 citations


Journal ArticleDOI
TL;DR: Biological active non-toxic gold nanoparticles green synthesized by a sulfite reductase of E. coli are potential candidates for application in different disciplines of medicine and biology from diagnostics to drug delivery systems, combating infectious diseases and anticancer therapies.

93 citations


Journal ArticleDOI
TL;DR: The laccase-glutaraldehyde-CF achieved excellent results in the clarification of apple juice, reducing 61 ± 1% of the original juice color and 29 ±- 1% of its turbidity, retaining up to 100% ofThe initial activity after a 10-times reuse assay.

90 citations


Journal ArticleDOI
TL;DR: Divinylsulfone activated agarose beads have been used to immobilize several lipases, those from Pseudomonas flourescens, Rhizomucor miehei, and Thermomyces lanuginosus, as well as the artificial chimeric phospholipase Lecitase Ultra, and shows that this support may be very useful for tuning lipase properties just by altering the first immobilization pH value.

82 citations


Journal ArticleDOI
TL;DR: In this article, the authors deal with the synthesis of efficient water soluble O-carboxymethyl chitosan (O-CMCS) based nanocomposites with nanostructured zinc oxide (n-ZnO) by ex-situ grafting method for the delivery of anticancer drug curcumin (Cr).

78 citations


Journal ArticleDOI
TL;DR: Maximum loaded Accurel-PS preparation permitted to improve the activity in this kinetic resolution compared to the PS commercial preparation by a 55-fold factor, and compared to Novozym 435 (the most active described in literature for this reaction) by a 23-fold factors.

77 citations


Journal ArticleDOI
TL;DR: The ability of the isolate to degrade petroleum sludge and the effect of its biosurfactant on biodegradation enhancement was tested and suggests the potential application in bioremediation of contaminated environment and microbial enhanced oil recovery.

Journal ArticleDOI
TL;DR: This review aims to provide the reader with an overview of the use of bio-based polymers, as well as their blends and composites, for tissue engineering and health care purposes, with special emphasis on supercritical fluid processing for drug delivery and scaffold fabrication.

Journal ArticleDOI
TL;DR: In this paper, the effect of ultrasound, thermal and alkali treatments on the characteristics of extracellular polymeric substances (EPSs) in activated sludge was investigated.

Journal ArticleDOI
TL;DR: Good's buffers ionic liquids (GB-ILs), composed of cholinium-based cations and Good's buffers anions, display self-buffering characteristics in the biological pH range, and their polarity and hydrophobicity can be easily tuned by a proper manipulation of their ions chemical structures.

Journal ArticleDOI
TL;DR: The irreversible immobilization permitted the reactivation of CALA biocatalysts inactivated by incubation in organic solvent, after unfolding in the presence of guanidine and refolding in aqueous buffer, and this strategy could not be applied to CRL.

Journal ArticleDOI
TL;DR: In this paper, the authors investigated the impact of multi freeze-thaw cycles on emulsifying activity and structural characteristics of soy protein isolate (SPI) and found that the emulsification activity index and emulsion stability index of the SPI submitted to 3, 4 and 5 freeze-thyaw cycles were significantly higher than that of native protein.

Journal ArticleDOI
TL;DR: In this article, the interactions of divalent metals (Ca2+ and Mg2+) with protein and polysaccharide components of extracellular polymeric substances (EPS) and deduce their role in imparting different physicochemical characteristics to aerobic granules in sequencing batch reactor (SBR) system.

Journal ArticleDOI
Fayin Zhu1, Lei Lu1, Shuai Fu, Xiaofang Zhong1, Mengzhu Hu1, Zixin Deng1, Tiangang Liu1 
TL;DR: The results demonstrate that the L3 fermentation process is easy scalable and that L3 could potentially replace the natural producer Blakeslea trispora in industrial production and could also be used to develop a highly efficient platform for overproduction of other terpenoids.

Journal ArticleDOI
TL;DR: Invertase belonging to glycoside hydrolases that catalyzes sucrose (table sugar) into two monosaccharides i.e. glucose and fructose assist in defense reaction against bacterial infections and gut fermentation by oxidation and categorize them as powerful antimicrobial and antioxidant agents.

Journal ArticleDOI
TL;DR: For the first time, high-throughput sequencing was employed to investigate the microbial communities of the biofilm in an autohydrogenotrophic denitrifying bio-ceramsite reactor, and it showed that the mainly reported aut HydrogenOTrophic den itrifying phyla Proteobacteria, Firmicutes and classes Gammaproteobacteria were all detected in the reactor, suggesting that this bio-cersite reactor presented better denitrification performance.

Journal ArticleDOI
TL;DR: In this article, the authors reported the biological synthesis of silver nanoparticles (AgNPs) using an aqueous extract of Pleurotus djamor var. roseus and its cytotoxicity against human prostate carcinoma (PC3) cells.

Journal ArticleDOI
TL;DR: In this paper, amino acid composition and electrophoretic patterns were observed in the obtained skin ASCs, but differences were found in the peptide maps of collagen digested by V8 protease, trypsin and lysyl endopeptidase.

Journal ArticleDOI
TL;DR: The results suggest the potential of k-AuNPs as a promising anti-cancer agent in breast cancer therapy.

Journal ArticleDOI
TL;DR: In this article, the optimum temperature of α-amylase was increased from 50 to 60°C and its optimum pH was shifted from 6 to 7 upon immobilization. But, the storage stability was not improved.

Journal ArticleDOI
TL;DR: In this paper, the authors used 2,3-dialdehyde cellulose (DAC) as a crosslinking agent to modify the physicochemical properties of silver carp collagen.

Journal ArticleDOI
TL;DR: In this article, the authors evaluated the composition, nutritional value, and potential bioactive peptides in hydrolysates obtained from different Atlantic salmon rest raw materials and evaluated the effect of enzymes (endogenous enzymes, Protamex, and papain plus bromelain), pretreatments, and industrial-compared to laboratory-scale production.

Journal ArticleDOI
TL;DR: In this paper, a Silkworm pupa (Bombyx mori ) protein (SPP) was treated by ultrasound, and then was hydrolyzed using alcalase.

Journal ArticleDOI
TL;DR: Preliminary results suggest the strong nutritional and functional potential of Tilapia by-product hydrolysates.

Journal ArticleDOI
TL;DR: To enhance the UDCA yield, the analytic yield of UDCA was significantly improved up to above 98% at a substrate load of 10 mM ( ca. 4 g L −1 ), without any detectable intermediate (7-oxo-LCA) as observed in the case of one-pot reaction.

Journal ArticleDOI
TL;DR: Results demonstrated that the multi-CLEA is a promising catalyst for biotechnological applications, including improved washing process and biodiesel production.