M
M. Caroline Müllenbroich
Researcher at European Laboratory for Non-Linear Spectroscopy
Publications - 4
Citations - 71
M. Caroline Müllenbroich is an academic researcher from European Laboratory for Non-Linear Spectroscopy. The author has contributed to research in topics: Light sheet fluorescence microscopy & Bessel beam. The author has an hindex of 3, co-authored 4 publications receiving 53 citations. Previous affiliations of M. Caroline Müllenbroich include National Research Council & University of Florence.
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Journal ArticleDOI
Bessel Beam Illumination Reduces Random and Systematic Errors in Quantitative Functional Studies Using Light-Sheet Microscopy
M. Caroline Müllenbroich,M. Caroline Müllenbroich,Lapo Turrini,Ludovico Silvestri,Ludovico Silvestri,Tommaso Alterini,Ali Gheisari,Natascia Tiso,Francesco Vanzi,Francesco Vanzi,Leonardo Sacconi,Leonardo Sacconi,Francesco S. Pavone,Francesco S. Pavone,Francesco S. Pavone +14 more
TL;DR: This work reports how Bessel beams reduce streaking artifacts and produce high-fidelity quantitative data demonstrating a fivefold increase in sensitivity to calcium transients and a 20- fold increase in accuracy in the detection of activity correlations in functional imaging.
Journal ArticleDOI
Comprehensive optical and data management infrastructure for high-throughput light-sheet microscopy of whole mouse brains
M. Caroline Müllenbroich,M. Caroline Müllenbroich,Ludovico Silvestri,Ludovico Silvestri,Leonardo Onofri,Leonardo Onofri,Irene Costantini,Marcel van ’t Hoff,Marcel van ’t Hoff,Leonardo Sacconi,Leonardo Sacconi,Giulio Iannello,Francesco S. Pavone +12 more
TL;DR: A confocal light-sheet microscope that has been optimized for three-dimensional imaging of structurally intact clarified whole-mount mouse brains and the mesoscale neuroanatomy imaged at micron-scale resolution in those datasets allows characterization and quantification of neuronal projections in unsectioned mouse brains.
Journal ArticleDOI
High-Fidelity Imaging in Brain-Wide Structural Studies Using Light-Sheet Microscopy.
M. Caroline Müllenbroich,Ludovico Silvestri,Antonino Paolo Di Giovanna,Giacomo Mazzamuto,Irene Costantini,Leonardo Sacconi,Francesco S. Pavone,Francesco S. Pavone +7 more
TL;DR: It is found that a third of the imaged volume of the brain was affected by strong striated image intensity inhomogeneity and a significant amount of information content lost with Gaussian illumination was accessible when interrogated with Bessel beams.
Proceedings ArticleDOI
High-fidelity imaging with Bessel-beam light-sheet microscopy for whole-brain structural and functional studies
M. Caroline Müllenbroich,Ludovico Silvestri,Lapo Turrini,Antonino Paolo Di Giovanna,Tommaso Alterini,Ali Gheisari,Leonardo Sacconi,Francesco Vanzi,Francesco S. Pavone +8 more
TL;DR: In this article, the authors present significant advantages of Bessel illumination in structural and functional imaging in light-sheet microscopy compared to conventional Gaussian beam illumination, and propose a method to use Bessel beam in light sheet microscopy.