High Throughput Isolation and Glycosylation Analysis of IgG–Variability and Heritability of the IgG Glycome in Three Isolated Human Populations

http://nathan.instras.com/documentDB/paper-196.pdf

Silica gel-based monoliths prepared by the sol-gel method: facts and figures.

Alternative bioseparation operations: life beyond packed-bed chromatography.

Rigid porous polymer monoliths are a new class of materials that emerged in the early 1990s. These monolithic materials are typically prepared using a simple molding process carried out within the confines of a closed mold. For example, polymerization of a mixture comprising monomers, free-radical initiator, and porogenic solvent affords macroporous materials with large through-pores that enable applications in a rapid flow-through mode. The versatility of the preparation technique is demonstrated by its use with hydrophobic, hydrophilic, ionizable, and zwitterionic monomers. Several system variables can be used to control the porous properties of the monolith over a broad range and to mediate the hydrodynamic properties of the monolithic devices. A variety of methods such as direct copolymerization of functional monomers, chemical modification of reactive groups, and grafting of pore surface with selected polymer chains is available for the control of surface chemistry. Since all the mobile phase must flow through the monolith, the convection considerably accelerates mass transport within the molded material, and the monolithic devices perform well, even at very high flow rates. The applications of polymeric monolithic materials are demonstrated mostly on the separations in the HPLC mode, although CEC, gas chromatography, enzyme immobilization, molecular recognition, advanced detection systems, and microfluidic devices are also mentioned.

Preparation and HPLC applications of rigid macroporous organic polymer monoliths

Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies.

Large-scale methacrylate monolithic columns: design and properties.

Methacrylate-based monoliths are formed during radical copolymerization as a consequence of the precipitation of polymeric chains from the reaction mixture, which consists of monomers, initiator, and the porogenic solvents. The effect of various methacrylate monomers on the porous structure of the monolith was investigated. Although the chemical structure of the monomers significantly affects the size of the pores and the porosity, the mechanism of pore formation in the case of the precipitation during polymerization is preserved. The porous structure was further correlated with the specific surface area, pressure drop, and dynamic binding capacity of the monoliths studied.

Methacrylate monoliths prepared from various hydrophobic and hydrophilic monomers – Structural and chromatographic characteristics

Characterisation of metal-chelate methacrylate monoliths.

Monoliths have already proven to be efficient chromatographic supports for the separation of various types of molecules. In this paper, the characterization of the novel reversed-phase support, CIM® RP-SDVB disk monolithic column is presented. Using a 3 mm long RP-SDVB disk monolithic column, excellent separation of proteins within a very short time was achieved. The pressure drop observed on the column was considerably low (few bars), even at flow rates of the mobile phase up to 30 mL/min. Due to the low pressure drop, the use of high flow rates was preferred since they did not influence the quality of the gradient separation. The separation of test proteins was performed within only 14 seconds; faster separations were limited by the configuration of the HPLC system. Furthermore, RP-SDVB disk monolithic columns were applied for fast separation of peptides. Five peptides of different lengths and composition were successfully separated in a very short time. Finally, the preparative purification on the labo...

M. Merhar

Papers

Large-scale methacrylate monolithic columns: design and properties.

Methacrylate monoliths prepared from various hydrophobic and hydrophilic monomers – Structural and chromatographic characteristics

Characterisation of metal-chelate methacrylate monoliths.

High performance reversed-phase liquid chromatography using novel cim RP-SDVB monolithic supports

Chapter 3 - Short Monolithic Columns Rigid Disks

M. Merhar

Papers

Large-scale methacrylate monolithic columns: design and properties.

Methacrylate monoliths prepared from various hydrophobic and hydrophilic monomers – Structural and chromatographic characteristics

Characterisation of metal-chelate methacrylate monoliths.

High performance reversed-phase liquid chromatography using novel cim RP-SDVB monolithic supports

Chapter 3 - Short Monolithic Columns ­ Rigid Disks

Chapter 3 - Short Monolithic Columns Rigid Disks