Book ChapterDOI
Chapter 3 - Short Monolithic Columns Rigid Disks
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This article is published in Journal of chromatography library.The article was published on 2003-12-31. It has received 9 citations till now.read more
Citations
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Journal ArticleDOI
High Throughput Isolation and Glycosylation Analysis of IgG–Variability and Heritability of the IgG Glycome in Three Isolated Human Populations
Maja Pučić,Ana Knezevic,Jana Vidič,Barbara Adamczyk,Mislav Novokmet,Ozren Polasek,Olga Gornik,Sandra Supraha-Goreta,Mark R. Wormald,Irma Redzic,Harry Campbell,Alan F. Wright,Nicholas D. Hastie,James F. Wilson,Igor Rudan,Manfred Wuhrer,Pauline M. Rudd,Djuro Josic,Djuro Josic,Gordan Lauc +19 more
TL;DR: The majority of the structural features of the IgG glycome were consistent with previous studies, but sialylation was somewhat higher than reported previously, indicating that the final glycan structures are not a simple result of competing enzymatic activities, but a carefully regulated outcome designed to meet the prevailing physiological needs.
Journal ArticleDOI
Alternative bioseparation operations: life beyond packed-bed chromatography.
TL;DR: The current state of unit operations posing as chromatography alternatives--including membrane filtration, aqueous two-phase extraction, three-phase partitioning, precipitation, crystallization, monoliths and membrane chromatography--and their potential to do the unthinkable are reviewed.
Journal ArticleDOI
Methacrylate‐based short monolithic columns: Enabling tools for rapid and efficient analyses of biomolecules and nanoparticles
Miloš Barut,Aleš Podgornik,Lidija Urbas,Boštjan Gabor,Peter Brne,Jana Vidič,Sašo Plevčak,Aleš Štrancar +7 more
TL;DR: The efficiency of the monolithic column is demonstrated--where the mass transfer between the stationary and mobile phase is greatly enhanced--for the in-process and final control of the new therapeutics.
Journal ArticleDOI
Glycidyl methacrylate and ethylhexyl acrylate based polyHIPE monoliths: Morphological, mechanical and chromatographic properties
Simona Jerenec,Mario Šimić,Aleš Savnik,Aleš Podgornik,Mitja Kolar,Marko Turnšek,Peter Krajnc +6 more
TL;DR: In this article, highly porous polymers (polyHIPEs) were prepared from glycidyl methacrylate (GMA) and ethylhexyl acrylate(EHA), their morphology investigated and mechanical and chromatographic characteristics evaluated.
Journal ArticleDOI
The 3D pore structure and fluid dynamics simulation of macroporous monoliths: High permeability due to alternating channel width.
Christian Jungreuthmayer,Petra Steppert,Gerhard Sekot,Armin Zankel,Herbert Reingruber,Jürgen Zanghellini,Alois Jungbauer +6 more
TL;DR: This study showed that the interconnection of small and wide pores is responsible for the excellent pressure flow properties ofPolymethacrylate-based monoliths and is a guide for further design of continuous porous materials to achieve good flow properties.
References
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Journal ArticleDOI
Continuous rods of macroporous polymer as high-performance liquid chromatography separation media
Journal ArticleDOI
Octadecylsilylated Porous Silica Rods as Separation Media for Reversed-Phase Liquid Chromatography
TL;DR: The performance of the silica rods was much better at a high flow rate than that of conventional columns packed with 5 μm C(18) silica particles having 12 and 30 nm pores, especially for high molecular weight species.
Journal ArticleDOI
High-performance liquid chromatography on continuous polymer beds
TL;DR: The experiments described in refs.
Journal ArticleDOI
Flow-through particles for the high-performance liquid chromatographic separation of biomolecules: perfusion chromatography.
Noubar B. Afeyan,Neal F. Gordon,István Mazsaroff,Laszlo Varady,S.P. Fulton,Yi Yang,Fred E. Regnier +6 more
TL;DR: It is shown that the rate of solute transport is strongly coupled to mobile phase Velocity such that bandspreading, resolution of proteins and dynamic loading capacity are unaffected by increases in mobile phase velocity up to several thousand centimeters per hour.
Journal ArticleDOI
Monolithic, “Molded”, Porous Materials with High Flow Characteristics for Separations, Catalysis, or Solid-Phase Chemistry: Control of Porous Properties during Polymerization
TL;DR: The porosity and flow characteristics of macroporous polymer monoliths that may be used to prepare separation media, flow-through reactors, catalysts, or supports for solid-phase chemistry can be controlled easily during their preparation.
Related Papers (5)
Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies.
Alois Jungbauer,Rainer Hahn +1 more