Journal ArticleDOI
Large-scale methacrylate monolithic columns: design and properties.
Aleš Podgornik,Janez Jančar,M. Merhar,S. Kozamernik,D. Glover,K. Čuček,Miloš Barut,Aleš Štrancar +7 more
Reads0
Chats0
TLDR
In this work, methacrylate-based monolithic columns with the bed volume up to 8000 ml are characterized and are found to be incompressible under these operating conditions and resistant to strong alkaline conditions.About:
This article is published in Journal of Biochemical and Biophysical Methods.The article was published on 2004-09-30. It has received 75 citations till now.read more
Citations
More filters
Journal ArticleDOI
Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies.
Alois Jungbauer,Rainer Hahn +1 more
TL;DR: The pressure drop characteristics, mass transfer properties, scale-up, and applications of monoliths in the context of conventional chromatography media are discussed.
Journal ArticleDOI
Characterization of a novel continuous supermacroporous monolithic cryogel embedded with nanoparticles for protein chromatography.
TL;DR: The protein adsorption capacity of the present cryogel bed was improved due to the embedded nano-sized solid adsorbents in the gel matrix, and the axial dispersion coefficient was found to be increased exponentially with the increase of liquid flow rate.
Journal ArticleDOI
Application of monoliths for plasmid DNA purification: Development and transfer to production
TL;DR: It could be shown, that up-scaling to the production scale using 800 ml CIM Convective Interaction Media radial flow monoliths is possible under low pressure conditions and CIM DEAE was successfully implemented as intermediate step of the cGMP pDNA manufacturing process.
Journal ArticleDOI
Preparation of methacrylate monoliths
E. G. Vlakh,Tatiana B. Tennikova +1 more
TL;DR: This review is an attempt to collect together the contributions of different groups working in the area of monolithic preparation, and examples of different methcrylate monomers and crosslinkers, as well as porogenic solvents, including polymer ones, used in monolith preparation are discussed.
Journal ArticleDOI
Applications of polymethacrylate-based monoliths in high-performance liquid chromatography.
E. G. Vlakh,Tatiana B. Tennikova +1 more
TL;DR: In this review, the applications of polymethacrylate-based monolithic columns are summarized for the separation, purification and analysis of low and high molecular mass compounds in the different HPLC formats, including micro- and large-scale HPLC modes.
References
More filters
Journal ArticleDOI
Longitudinal diffusion and resistance to mass transfer as causes of nonideality in chromatography
TL;DR: In this article, a rate theory for band broadening in linear, non-ideal chromatography is presented, wherein nonideality is caused by axial molecular diffusion, axial eddy diffusion, and finiteness of transfer coefficient.
Journal ArticleDOI
Monolithic stationary phases for liquid chromatography and capillary electrochromatography.
TL;DR: A monolithic stationary phase is the continuous unitary porous structure prepared by in situ polymerization or consolidation inside the column tubing and the surface is functionalized to convert it into a sorbent with the desired chromatographic binding properties.
Journal ArticleDOI
High-performance membrane chromatography: Highly efficient separation method for proteins in ion-exchange, hydrophobic interaction and reversed-phase modes
TL;DR: In this paper, the effects of process variables such as concentration of displacement agent, flow-rate and gradient slope on HPMC separations in the ion exchange, hydrophobic interaction and reversed phase modes were studied using model protein mixtures.
Journal ArticleDOI
Construction of large-volume monolithic columns.
TL;DR: A heat release during the polymerization is analyzed and a mathematical model is derived for the prediction of the maximal thickness of the monolithic annulus having a uniform structure for GMA-EDMA monoliths.
Book ChapterDOI
Short Monolithic Columns as Stationary Phases for Biochromatography
TL;DR: Short Monolithic Columns (SMC) were engineered to combine both features and have the potential of becoming the method of choice for the purification of larger biomolecules and nanopartides on the semi-preparative scale.
Related Papers (5)
Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies.
Alois Jungbauer,Rainer Hahn +1 more