Showing papers by "M. Revanasiddappa published in 2015"

Analytical Stability Indicative Method Development and Validation by High Pressure Liquid Chromatography for Assay in Ciprofloxacin hydrochloride Drug Substances

Abstract: Ciprofloxacin is an antibiotic generic drug that can treat a number of bacterial infections. It is a second generic fluoroquinolone. This work presents a simple, sensitive and stability indicative method for assay determination of drug substances ciprofloxacin hydrochloride by high pressure liquid chromatography (HPLC). Separation of impurities from the main drug substances and accurate assay quantification with a simple method is difficult by Spectrophotometry. The separation of co-eluents and impurities from the ciprofloxacin drug substances was archived by HPLC with simple mobile phase 0.15% orthophosphoric acid adjusted pH to 3.0 with triethylamine and acetonitrile using a gradient program, column inertsil C18, 250 mm × 4.0 mm, 5 μm. Flow rate was 0.7 mL/min, the column temperature and injection volume were 35°C and 10.0 μL. Chromato-graphic analysis was carried out at wavelength 278 nm. The developed method was validated according to the international conference on harmonization (ICH) guidelines regarding: Precision, specificity by degradation, linearity, accuracy, range and robustness. The proposed method showed good linearity (correlation coefficient and regression coefficient were not less than 0.999 and 0.998) in the range of 50% to 150% of working concentration. The recovery at 50%, 100% and 150% of working concentration level was within 98.0% to 102.0%. The range of the method is concluded that developed method is from 50% to 150% of target concentration for ciprofloxacin hydrochloride. The applicability of the proposed method was verified through the analysis of the samples and percentage recoveries in the range of 98% to 102% were obtained without any interference detected at the main peak in the chromatogram. The validated method is highly selective, simple, accurate, cost effective, and it is applicable for stability studies and routine quality-control analysis in the pharmaceutical industries.

Microwave Synthesis, Spectroscopic, Thermal and Biological Studies of some Transition Metal Complexes containing Heterocyclic Ligand

Abstract: The microwave (MW) assisted synthesis of coordination metal complexes is most important technique, because of reducing chemical waste and reaction times in various chemical transformations. In the present study, some new Schiff base metal complexes of ZrO(II), VO(II), Cr(III), Mn(II), Fe(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II) and Hg(II) derived from o-vanillin with 6-(trifluoro methoxy) benzothiozole-2-amine have been synthesised by using microwave radiation. Obtained coordination metal complexes were characterized with the help of several physical methods such as elemental analysis, FT-IR, molar conductance, electronic spectra, 1H-NMR, ESR, magnetic susceptibility, thermal, electrical conductivity and XRD analysis. All these data reveals the coordination number were 4, 5 and 6. Elemental analysis indicates that the complexes are of the type 1:1, 1:2 and 1:3 (M: L). The 1H-NMR spectral data attribute that the phenolic protons have been displaced during the complexation. The thermo gravimetric analysis shows that the presence of water molecule in the coordination metal complexes, which confirmed the loss of water molecule in the first step; followed by decomposition of ligand in the subsequent step. The ligand and coordination metal complexes were also tested against the Gram-positive bacteria; E-coli, Salmonella and Klebshilla, Gram-negative bacteria; S.aurioes and Streptococci.

Synthesis, Characterization, Antimicrobial Activity, Antifungal Activity and DNA Cleavage Studies of Transition Metal Complexes with Schiff Base Ligand

Abstract: Transition metal complexes of Cu(II), Co(II), Ni(II) and Zn(II) have been synthesized with the Schiff base ligand prepared by the condensation reaction between isooxazol-3-yl amine and salicylaldehyde. Elemental analysis of these complexes suggest that these metal ions forms complexes of type ML2(H2O)2 stoichiometry for Cu(II), Co(II), Ni(II) and Zn(II). The ligand behaves as bidentate and forms coordinate bonds through O and N atoms. Magnetic susceptibility, IR, UV – Visible, Mass and ESR spectral studies suggest that Cu(II), Co(II), Ni(II) and Zn(II) complexes posses octahedral geometry. The complexes were tested for their antimicrobial activity against the bacterial strains Staphylococcus aureus and Bacillus subtilis. The Schiff base metal complexes evaluated for their antifungal activity against the fungi Aspergillus niger and Cladosporium oxysporum. The DNA cleavage studies of Schiff base complexes were studied by agarose gel electrophoresis method using Calf – Thymus DNA.