M. Riad

TL;DR: The highest density of immunostaining was found in limbic areas (lateral septum, CA1 area of Ammon's horn and dentate gyrus in the hippocampus, and frontal and entorhinal cortices), in agreement with previous autoradiographic studies with selective radioligands showing the enrichment of these regions in serotonin1A receptor binding sites.

TL;DR: Polyclonal antibodies were raised by the repeated injection of rabbits with a synthetic peptide corresponding to a highly selective portion of the amino acid sequence of the rat 5-HT1A receptor to allow the immunoprecipitation of 5-ht1A receptors but not of other 5- HT1 sites solubilized from rat hippocampal membranes.

TL;DR: It is demonstrated that 5-HT1A receptors are synthesized by serotoninergic neurons in the dorsal raphe nucleus, and by neurons located postsynaptically with regard to serotoninergic projections in other areas, as well as consistent with the absence of 5- HT1A receptor up regulation reported under this condition.

TL;DR: 5-HT can also indirectly influence the differentiation of serotonergic neurons by the intermediate of astrocytes, and it was shown in the laboratory that 5-HT1A agonists can stimulate the cholinergic parameters of primary cultures of rat fetal septal neurons.

TL;DR: Repeated treatment of primary cultures of fetal rat septal neurons with 5-HT1A receptor agonists (8-OH-DPAT, ipsapirone, gepirone and buspirone) increased choline acetyltransferase activity after 6-7 days in culture, suggesting that under in vitro conditions ipsAPirone may exert a direct trophic action on sePTal cholinergic neurons.