Transesterification of Jatropha oil was carried out in t-butanol solvent using immobilized lipase from Enterobacter aerogenes. The presence of t-butanol significantly reduced the negative effects caused by both methanol and glycerol. The effects of various reaction parameters on transesterification of Jatropha oil were studied. The maximum yield of biodiesel was 94% (of which 68% conversion was achieved with respect to methyl oleate) with an oil:methanol molar ratio of 1:4, 50 U of immobilized lipase/g of oil, and a t-butanol:oil volume ratio of 0.8:1 at 55°C after 48 h of reaction time. There was negligible loss in lipase activity even after repeated use for seven cycles. To the best of our knowledge this is the first report on biodiesel synthesis using immobilized E. aerogenes lipase.

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Enzymatic transesterification of Jatropha oil

Enzymatic biodiesel synthesis – Key factors affecting efficiency of the process

Biodiesel production using enzymatic transesterification – Current state and perspectives

tert-Butanol, as a novel reaction medium, has been adopted for lipase-catalyzed transesterification of rapeseed oil for biodiesel production, with which both the negative effects caused by excessive methanol and by-product glycerol could be eliminated. Combined use of Lipozyme TL IM and Novozym 435 was proposed further to catalyze the methanolysis and the highest biodiesel yield of 95% could be achieved under the optimum conditions (tert-butanol/oil volume ratio 1:1; methanol/oil molar ratio 4:1; 3% Lipozyme TL IM and 1% Novozym 435 based on the oil weight; temperature 35 °C; 130 rpm, 12 h). There was no obvious loss in lipase activity even after being repeatedly used for 200 cycles with tert-butanol as the reaction medium. Furthermore, waste oil was also explored for biodiesel production and it has been found that lipase also showed good stability in this novel system.

Lipase-catalyzed transesterification of rapeseed oils for biodiesel production with a novel organic solvent as the reaction medium

As our fossil raw materials irrevocably diminish and environmental pressures build, a progressive changeover of chemical industries to renewable feedstocks emerges as an inevitable necessity. Experts realistically predict the end of cheap oil in 2040 at the latest, a development that we can already witness as chemical manufacturers confront the rising cost of oil and natural gas. The transition to a more biobased production system is currently underway, and much attention has been given to the catalytic conversion of renewable feedstocks and chemicals. The conversion of renewable materials to hydrogen assists in the utilization of renewable energy sources, and conversion to commodity chemicals facilitates the replacement of petroleum by renewable resources. Recently, it has been proposed that renewable resources such as plant-derived sugars and other compounds be used to synthesize the compounds necessary for the production of pharmaceuticals, agricultural chemicals, plastics, and transportation fuels. Compounds that are now derived from fossil resources might be produced in future biorefineries.

Commodity Chemicals Derived from Glycerol, an Important Biorefinery Feedstock

1-butyl oleate synthesis by immobilized lipase from Rhizopus oryzae: a comparative study between n-hexane and solvent-free system

Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system

Production of mono-olein by immobilized Staphylococcus simulans lipase in a solvent-free system: Optimization by response surface methodology

Immobilization studies and biochemical properties of free and immobilized Rhizopus oryzae lipase onto CaCO3: a comparative study.

Improved antioxidant activity and oxidative stability of spray dried European eel (Anguilla anguilla) oil microcapsules: Effect of emulsification process and eel protein isolate concentration

Maha Karra-Chaâbouni

Papers

1-butyl oleate synthesis by immobilized lipase from Rhizopus oryzae: a comparative study between n-hexane and solvent-free system

Production of isoamyl acetate with immobilized Staphylococcus simulans lipase in a solvent-free system

Production of mono-olein by immobilized Staphylococcus simulans lipase in a solvent-free system: Optimization by response surface methodology

Immobilization studies and biochemical properties of free and immobilized Rhizopus oryzae lipase onto CaCO3: a comparative study.

Improved antioxidant activity and oxidative stability of spray dried European eel (Anguilla anguilla) oil microcapsules: Effect of emulsification process and eel protein isolate concentration