Diagnostic criteria and classification of mastocytosis: a consensus proposal

Most consensus leukemia & lymphoma antibody panels consist of lists of markers based on expert opinions, but they have not been validated. Here we present the validated EuroFlow 8-color antibody panels for immunophenotyping of hematological malignancies. The single-tube screening panels and multi-tube classification panels fit into the EuroFlow diagnostic algorithm with entries defined by clinical and laboratory parameters. The panels were constructed in 2–7 sequential design–evaluation–redesign rounds, using novel Infinicyt software tools for multivariate data analysis. Two groups of markers are combined in each 8-color tube: (i) backbone markers to identify distinct cell populations in a sample, and (ii) markers for characterization of specific cell populations. In multi-tube panels, the backbone markers were optimally placed at the same fluorochrome position in every tube, to provide identical multidimensional localization of the target cell population(s). The characterization markers were positioned according to the diagnostic utility of the combined markers. Each proposed antibody combination was tested against reference databases of normal and malignant cells from healthy subjects and WHO-based disease entities, respectively. The EuroFlow studies resulted in validated and flexible 8-color antibody panels for multidimensional identification and characterization of normal and aberrant cells, optimally suited for immunophenotypic screening and classification of hematological malignancies.

/pdf/euroflow-antibody-panels-for-standardized-n-dimensional-flow-32igoftjs0.pdf

EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes.

Although a classification for mastocytosis and diagnostic criteria are available, there remains a need to define standards for the application of diagnostic tests, clinical evaluations, and treatment responses. To address these demands, leading experts discussed current issues and standards in mastocytosis in a Working Conference. The present article provides the resulting outcome with consensus statements, which focus on the appropriate application of clinical and laboratory tests, patient selection for interventional therapy, and the selection of appropriate drugs. In addition, treatment response criteria for the various clinical conditions, disease-specific symptoms, and specific pathologies are provided. Resulting recommendations and algorithms should greatly facilitate the management of patients with mastocytosis in clinical practice, selection of patients for therapies, and the conduct of clinical trials.

https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2362.2007.01807.x

Standards and standardization in mastocytosis: consensus statements on diagnostics, treatment recommendations and response criteria.

https://redbook.streamliners.co.nz/mastocytosis_review_metcalfe_blood2008.pdf

Mast cells and mastocytosis

Mastocytosis: 2016 updated WHO classification and novel emerging treatment concepts

Indolent Systemic Mast Cell Disease in Adults: Immunophenotypic Characterization of Bone Marrow Mast Cells and Its Diagnostic Implications

The following text presents the results of a content analysis study focusing on the image of immigration in Spanish prime-time television fiction with Cultivation theory as a reference. Two representative samples were recorded in 2011, out of the six main national channels. The analysis derived from the analysis of these fiction programs (series and films) derived provided results showing that: (1) Immigrant/foreign characters were underrepresented when taking into account real [14] data; (2) Immigrant/foreign characters were usually presented in nonprotagonist (mainly antagonist or villain) narrative roles; and (3) Socio-demographic features of immigrant/foreign characters such as their education, socio-economic status, or occupation tended to entail lower levels than those in natives. The results are discussed considering the importance of the reduction of prejudice in television fiction.

Marı́a A. Garcı́a Marcos

Papers

Indolent Systemic Mast Cell Disease in Adults: Immunophenotypic Characterization of Bone Marrow Mast Cells and Its Diagnostic Implications

Portraying immigration in Spanish prime-time TV fiction