Maria Grazia Cotticelli

TL;DR: A 200-300 kb region of chromosome 3p14.2, including the fragile site locus FRA3B, is homozygously deleted in multiple tumor-derived cell lines, allowing identification of the human FHIT gene, a member of ther histidine triad gene family, which encodes a protein with 69% similarity to an S. pombe enzyme.

TL;DR: To determine the role of the FHIT gene, which encompasses the fragile site at 3p14.2, tumors of the small cell and non-small cell type and cell lines were analyzed by reverse transcription of FHit mRNA, followed by PCR amplification and sequencing of products, suggesting a critical role in lung carcinogenesis.

TL;DR: The FHIT gene, encoded by 10 exons in a 1.1-kb transcript, encompasses approximately 1 Mb of genomic DNA, which includes the hereditary RCC t(3;8) translocation break at 3p14.2, the FRA3B common fragile region, and homozygous deleting in various cancer-derived cell lines.

TL;DR: The sizes of the homozygous deletions in a number of cancer cell lines are determined after isolation of a yeast artificial chromosome contig and development of STS markers which fall between D3S1234 and D2S1481, which flank the deletions.

TL;DR: It is concluded that the murine Fhit gene, like its human counterpart, is a target of alterations involved in murine carcinogenesis.