Showing papers by "Mariusz Kaczmarczyk published in 2014"

Does the A9285g Polymorphism in Collagen Type XII α1 Gene Associate with the Risk of Anterior Cruciate Ligament Ruptures

Abstract: One of the most severe injuries sustained by athletes is rupture of the anterior cruciate ligament (ACL). Recent investigations suggest that a predisposition for ACL rupture may be the result of specific genetic sequence variants. In light of this, we decided to investigate whether the COL12A1 A9285G polymorphism was associated with ACL ruptures in Polish football players. We compared genotypic and allelic frequencies of the COL12A1 A9285G polymorphism in two groups of athletes: 91 male football players (23 ± 3 years) with surgically diagnosed primary ACL ruptures who qualified for ligament reconstruction (cases) and 143 apparently healthy, male football players of the same ethnicity, a similar age category, and a comparable level of exposure to ACL injury, who were without any self-reported history of ligament or tendon injury (controls). DNA samples extracted from the oral epithelial cells were genotyped by using a real-time polymerase chain reaction (Ri-Ti-PCR) method. The genotype distribution in the cases were not different from those in controls (p = 0.70). The frequency of the G allele was lower in the cases (18.1%) but not statistically significant (p = 0.40) when compared with controls (21.3%). Our results are in contradiction to the hypothesis that the COL12A1 A9285G polymorphism is associated with a predisposition for ACL injury. However, these conclusions should be supported with more experimental studies on COL12A1 polymorphisms.

Association of the MTHFR 1298A>C (rs1801131) polymorphism with speed and strength sports in Russian and Polish athletes.

Abstract: It has been suggested that DNA hypomethylation because of poorer effectiveness of the 5,10-methylenetetrahydrofolate reductase (MTHFR) enzyme induces muscular growth. We hypothesised that the common, functional 1298A>C polymorphism in the MTHFR gene is associated with athletic status. To test this hypothesis, we investigated the distribution of the 1298A>C variant in Polish (n = 302) and Russian (n = 842) athletes divided into four groups: endurance, strength-endurance, sprint-strength and strength-endurance, as well as in 1540 control participants. We found different genotypes (the AC heterozygote advantage) and allele distributions among sprint-strength athletes and strength athletes than the groups of sedentary controls for each nationality. In the combined study, the allelic frequencies for the 1298C variant were 35.6% in sprint-strength athletes (OR 1.18 [1.02–1.36], P = 0.024 vs. controls) and 38.6% in strength athletes (OR 1.34 [1.10–1.64], P = 0.003 vs. controls). The results of the initia...

Interaction Between ACE I/D and ACTN3 R557X Polymorphisms in Polish Competitive Swimmers

Abstract: We hypothesized that the ACE ID / ACTN3 R577X genotype combination was associated with sprint and endurance performance Therefore, the purpose of the present study was to determine the interaction between both ACE ID and ACTN3 R577X polymorphisms and sprint and endurance performance in swimmers Genomic DNA was extracted from oral epithelial cells using GenElute Mammalian Genomic DNA Miniprep Kit (Sigma, Germany) All samples were genotyped using a real-time poly- merase chain reaction The ACE I/D and the ACTN3 R577X genotype frequencies met Hardy-Weinberg expectations in both swimmers and controls When the two swimmer groups, long distance swimmers (LDS) and short distance swimmers (SDS), were compared with control subjects in a single test, a significant association was found only for the ACE polymorphism, but not for ACTN3 Additionally, four ACE/ACTN3 combined genotypes (ID/RX, ID/XX, II/RX and II/XX) were statistically significant for the LDS versus Control comparison, but none for the SDS versus Control comparison The ACE I/D and the ACTN3 R577X polymorphisms did not show any association with sprint swimming, taken individually or in combination In spite of numerous previous reports of associations with athletic status or sprint performance in other sports, the ACTN3 R577X polymorphism, in contrast to ACE I/D, was not significantly associated with elite swimming status when considered individually However, the combined analysis of the two loci suggests that the co-occurrence of the ACE I and ACTN3 X alleles may be beneficial to swimmers who compete in long distance races

The Pro12Ala Polymorphism of the Peroxisome Proliferator-Activated Receptor Gamma Gene Modifies the Association of Physical Activity and Body Mass Changes in Polish Women

Abstract: Peroxisome proliferator-activated receptor γ is a key regulator of adipogenesis, responsible for fatty acid storage and maintaining energy balance in the human body. Studies on the functional importance of the PPARG Pro12Ala polymorphic variants indicated that the observed alleles may influence body mass measurements; however, obtained results were inconsistent. We have decided to check if body mass changes observed in physically active participants will be modulated by the PPARG Pro12Ala genotype. The genotype distribution of the PPARG Pro12Ala allele was examined in a group of 201 Polish women measured for selected body mass variables before and after the completion of a 12-week training program. The results of our experiment suggest that PPARG genotype can modulate training-induced body mass measurements changes: after completion of the training program, Pro12/Pro12 homozygotes were characterised by a greater decrease of body fat mass measurements in comparison with 12Ala allele carriers. These results indicate that the PPARG 12Ala variant may impair the training-induced positive effects on body mass measurements; however, the detailed mechanism of such interaction remained unclear and observed correlation between PPARG genotype and body mass differential effects should be interpreted with caution.

THE GSTP1 c.313A>G POLYMORPHISM MODULATES THE CARDIORESPIRATORY RESPONSE TO AEROBIC TRAINING.

Abstract: The GSTP1 c.313A>G polymorphism is a candidate to explain some of the individual differences in cardiorespiratory fitness phenotypes' responses to aerobic exercise training. We aim to explore the association between the GSTP1 c.313A>G polymorphism and the response to low-high impact aerobic exercise training. Sixty-six Polish Caucasian women were genotyped for the GSTP1 c.313A>G polymorphism; 62 of them completed 12-week aerobic (50-75% HRmax) exercise training and were measured for selected somatic features (body mass and BMI) and cardiorespiratory fitness indices - maximal oxygen uptake (VO 2max, maximum heart rate (HRmax), maximum ventilation (VEmax) and anaerobic threshold (AT) - before and after the training period. Two-factor analysis of variance revealed a main training effect for body mass reduction (p=0.007) and BMI reduction (p=0.013), improvements of absolute and relative VO2max (both p G interaction was found only for FFM (p=0.042), absolute and relative VO2max (p=0.029 and p=0.026), and VEmax (p=0.005). As the result of training, significantly greater improvements in VO 2max, VEmax and FFM were gained by the GG+GA group compared to the AA genotype group. The results support the hypothesis that heterogeneity in individual response to training stimuli is at least in part determined by genetics, and GSTP1 c.313A>G may be considered as one (of what appear to be many) target polymorphisms to influence these changes.

Possible association of ABCB1:c.3435T>C polymorphism with high-density-lipoprotein-cholesterol response to statin treatment--a pilot study.

Abstract: The gene product ABCB1 (formerly MDR1 or P-glycoprotein) is hypothesized to be involved in cholesterol cellular trafficking, redistribution and intestinal re-absorption. Carriers of the ABCB1 :3435T allele have previously been associated with decreases in ABCB1 mRNA and protein concentrations and have been correlated with changes in serum lipid concentrations. The aim of this study was to investigate possible association between the ABCB1 :3435T>C polymorphism and changes in lipids in patients following statin treatment. Outpatients (n=130) were examined: 43 men (33%), 87 women (67%): treated with atorvastatin or simvastatin (all patients with equivalent dose of 20 or 40 mg/d simvastatin). Blood was taken for ABCB1 :3435T>C genotyping , and before and after statin treatment for lipid concentration determination (total cholesterol, high-density-lipoprotein-cholesterol (HDL-C), triglycerides). Change (Δ) in lipid parameters, calculated as differences between measurements before and after treatment, were analyzed with multiple regression adjustments: gender, diabetes, age, body mass index, equivalent statin dose, length of treatment. Univariate and multivariate analyses showed significant differences in ΔHDL-C (univariate p=0.029; multivariate p=0.036) and %ΔHDL-C (univariate p=0.021; multivariate p=0.023) between patients with TT (-0.05 ± 0.13 g/l; -6.8% ± 20%; respectively) and CC+CT genotypes (0.004 ± 0.15 g/l; 4.1 ± 26%; respectively). Reduction of HDL-C in homozygous ABCB1 :3435TT patients suggests this genotype could be associated with a reduction in the benefits of statin treatment.

PTPN22 1858C>T gene polymorphism in patients with SLE: association with serological and clinical results.

Abstract: To assess the association between PTPN22 1858C>T gene polymorphism and susceptibility to, and clinical presentation of, systemic lupus erythematosus (SLE). Our study included 135 SLE patients (120 women and 15 men; mean age 45.1 years; mean course of disease from 0.5 to 31 years) and 201 healthy subjects. The PTPN22 1858C>T gene polymorphism was genotyped by polymerase chain reaction restriction fragment length polymorphism. A significantly higher incidence of genotype CT in patients with SLE (36.3 %) was found, compared with the control group (24.9 %). The frequencies of C1858 and T1858 alleles were 78.1 and 21.9 % in SLE patients and 86.1 and 13.9 % in controls, respectively. Significantly higher SLE susceptibility was observed in patients carrying at least one T allele (p = 0.009; OR 1.86; 95 % CI 0.14–3.05). Significant association of the PTPN22 T1858 allele (CT + TT vs.CC) and secondary antiphospholipid syndrome was observed (p = 0.049). In SLE patients carrying the T1858 allele, higher levels of antiphospholipid antibodies (anticardiolipin antibodies and/or lupus anticoagulant) were found (p = 0.030; OR 2.17; 95 % CI 1.07–4.44).

Influence of selected factors on long-term kidney graft survival--a multivariable analysis.

Abstract: Background Long-term function of transplanted kidney is the factor determining quality of life for transplant recipients. The aim of this study was to evaluate the effect of selected factors on time of graft function after renal transplantation within 15 years of observation. Methods Preoperative and intraoperative factors were analyzed in 232 kidney recipients within a 15-year observation period. Analysis included age, sex, cause of recipient's renal failure, length of hemodialyses before transplantation, peak panel reactive antibodies test, human leukocyte antigen compatibility, cold ischemia time, delayed graft function occurrence, length and time of hemodialyses after transplantation, early graft rejection, creatinine level at days 1, 3, 7, 30, 90, and 180 after transplantation, and influence of these factors on the time of graft function. Statistical analysis was performed with the use of univariate and multivariate Kaplan-Meier test and Cox regression proportional hazards model, with P Results Univariate analysis showed significantly shorter renal graft function in the group of recipients with higher creatinine levels in all of the analyzed time periods and in patients experiencing delayed graft function. Length of time of hemodialyses after transplantation and number of dialyses had significant impact on worsening of late transplant results. Multivariate analysis reported that early graft rejection in the postoperative period is an independent factor improving late graft function: P = .002; hazard ratio (HR), 0.49 (95% confidence interval [CI], 0.31–0.78). Higher creatinine level at day 90 after kidney transplantation is a predictive factor of late graft dysfunction: P = .002; HR, 1.68 (95% CI 1.2–2.35). Conclusions Creatinine level at day 90 after renal transplantation is the prognostic factor of long-term kidney function. Early transplant rejection leads to introduction of more aggressive immunosuppression protocol, which improves long-term transplant results.

Endothelial nitric oxide synthase g894t (rs1799983) gene polymorphism in polish athletes

Abstract: The NOS3 gene has been associated with athletic endurance performance and elite power athletic status. With respect to NOS3 G894T and its relation to athletic performance or status, results across various studies have not been consistent. Therefore, the lack of consistency among previous studies prompted us to design a case-control study in a Polish Caucasian population to examine the relationship between the NOS3 G894T polymorphism and athletes' status, i.e. type and intensity of exercise performed (power- oriented, "mixed" power/endurance activity, endurance-oriented) and the possible association between the G894T variant and athletic performance. The case-control study was performed in a group of 360 Polish athletes (cases) of the highest nationally competitive standard (male n=156 and female n=67) and 191 unrelated, sedentary control subjects. The G894T genotype and allele distributions differed significantly between power-oriented (P=0.009, P=0.003), "mixed" (P=0.021, P=0.009), endurance (P=0.043, P=0.014) athletes when compared to control subjects (P values for genotypes and alleles, respectively). There were no significant differences between elite and sub-elite athletes in any group. The over-representation of the GG genotype and G allele in all athletes suggests that the G894 allele may favour all types of sports, however, the strongest predisposition was seen among power-oriented athletes.

Effect of recipient sensitization (peak panel reactive antibodies) on 15-year survival after kidney transplantation.

Abstract: Background Despite dynamic development within the field of transplantology, the immunization of a potential organ recipient remains an important issue for transplant teams. Panel reactive antibodies (PRA) identification in the serum of the recipient remains routine practice in the majority of transplantation centers. The influence of peak PRA levels on graft function is a well known fact. The aim of this study was to determine the effect of peak PRA on long-term survival after renal transplantation. Methods The study was conducted on a group of 232 kidney recipients from multiorgan donors, transplanted in 6 transplant centers in Poland from 1995 to 1997. Data analyzed in this study included recipients' age, sex, PRA, HLA, number and time of hemodialyses after the transplantation, cold ischemia time, and etiology of end-stage renal disease. The effect of data examined in this study on mortality was evaluated at set time points at 5, 10, and 15 years after transplantation. The statistical methods included monofactorial and multifactorial Kaplan-Meier survival analysis and Cox proportional hazards model for mortality prediction. A P value of <.05 was considered to be statistically significant. Results Among all of the analyzed factors, only peak PRA concentrations significantly correlated with increased mortality among renal transplant recipients. The results were analyzed in all of the set time points: P = .007 at 5 years, P = .014 at 10 years, and P = .05 at 15 years after transplantation. Conclusions The increased level of PRA in kidney recipients is a risk factor increasing mortality after the transplantation.

Jakość życia pacjentki z toczniem rumieniowatym układowym - opis przypadku = Quality of life patient with systemic lupus erythematosus - a case report

Abstract: Daniluk Aleksandra Eliza, Stepien-Slodkowska Marta, Kaczmarczyk Mariusz. Jakośc zycia pacjentki z toczniem rumieniowatym ukladowym - opis przypadku = Quality of life patient with systemic lupus erythematosus - a case report. Journal of Health Sciences. 2014;4(13):26-32. ISSN 1429-9623 / 2300-665X. http://www.journal.rsw.edu.pl/index.php/JHS/article/view/2014%3B4%2813%29%3A26-32 http://www.ojs.ukw.edu.pl/index.php/johs/article/view/2014%3B4%2813%29%3A26-32 https://pbn.nauka.gov.pl/works/491449 DOI: 10.5281/zenodo.13133 http://dx.doi.org/10.5281/zenodo.13133 The former journal has had 5 points in Ministry of Science and Higher Education of Poland parametric evaluation. Part B item 1107. (17.12.2013). © The Author (s) 2014; This article is published with open access at Licensee Open Journal Systems of Radom University in Radom, Poland Open Access. This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. This is an open access article licensed under the terms of the Creative Commons Attribution Non Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non commercial use, distribution and reproduction in any medium, provided the work is properly cited. Conflict of interest: None declared. Received: 10.09.2014. Revised 15.09.2014. Accepted: 04.11.2014. Jakośc zycia pacjentki z toczniem rumieniowatym ukladowym - opis przypadku Quality of life patient with systemic lupus erythematosus - a case report Aleksandra Eliza Daniluk Marta Stepien-Slodkowska Mariusz Kaczmarczyk Wydzial Kultury Fizycznej i Promocji Zdrowia, Uniwersytet Szczecinski Faculty of Physical Education and Health Promotion, The University of Szczecin Slowa kluczowe: toczen rumieniowaty ukladowy, jakośc zycia, kwestionariusz MOS - SF-36 Keywords: systematic lupus erythematosus, quality of life, questionnaire MOS - SF-36 Streszczenie Wprowadzenie. Toczen rumieniowaty ukladowy (systematic lupus erythematosus – SLE) jest chorobą powstającą na podlozu autoimmunizacji w wyniku, ktorej dochodzi do uszkodzenia narządow i ukladow organizmu. Dotyka najcześciej kobiet w przedziale wiekowym 15 – 40 lat. Roznorodnośc objawow klinicznych i stan zdrowia fizyczny, psychiczny oraz spoleczny w poszczegolnych fazach choroby warunkuje jakośc zycia pacjentow. Cel badan. Celem niniejszej pracy byla ocena jakości zycia pacjentki z rozpoznanym toczniem rumieniowatym ukladowym, poprzez zastosowanie polskiej wersji kwestionariusza SF-36 oraz ocena jej stanu zdrowia fizycznego i psychicznego. Material i metoda badan. Material do badan stanowily dane uzyskane w 2014 roku od kobiety w wieku 21 lat chorującej na toczen rumieniowaty ukladowy. U badanej stwierdzono 6 objawow wystepowania choroby spośrod 11 podanych przez Amerykanskie Towarzystwo Reumatologiczne. Metodą zastosowaną w badaniu byla metoda indywidualnych przypadkow. Techniki badawcze wykorzystywane do pozyskania danych to wywiad, badanie dokumentow i ankieta, w ktorej narzedziem byl kwestionariusz SF-36 oceniający jakośc zycia pacjentki. Opis przypadku. Zastosowany kwestionariusz MOS - SF-36 w polskiej wersji okazal sie przydatny do oceny jakości zycia pacjentki z SLE. W chwili badania ogolna jakośc zycia kobiety byla dobra Podsumowanie. Zadowalająca okazala sie sprawnośc fizyczna badanej. Stwierdzono brak ograniczen w funkcjonowaniu związanych z somatycznym i psychicznym stanem zdrowia. Najbardziej negatywny wplyw na jakośc zycia pacjentki wywolywal czesto pojawiający sie bol, obawa przed pogorszeniem stanu zdrowia i niekorzystnymi diagnozami lekarskimi podczas czestych wizyt kontrolnych. Abstract Introduction. Systemic lupus erythematosus (lupus erythematosus - SLE) is a systemic autoimmunity disease that can affect any part of the organ or body. It most commonly affects women between the ages of 15 and 40 years. The variety of symptoms and the physical, mental and social health in different phases of the disease determines the quality of life of patients. Purpose of the study. The aim of this study was to assess the quality of life of the patient diagnosed with systemic lupus erythematosus, by the use of the Polish version of the SF-36 questionnaire and the assessment of her state of physical and mental health. Materials and methods. The study material consisted data obtained in 2014 from a woman at the age of 21 suffering from systemic lupus erythematosus. The woman was diagnosed with 6 out of 11 symptoms of the disease given by the American College of Rheumatology. The method used in the study was the method of individual cases. Research techniques used to obtain data were the following: the interview and the study of documents and survey, in which the instrument was a questionnaire SF-36 evaluating the quality of life of the patient. Case . The questionnaire MOS - SF-36 used in the Polish version proved to be useful for assessing the quality of life of the patient with SLE. At the time of the study the overall quality of a woman's life was good. Summary . The physical function turned out to be satisfactory. One stated lack of limitations in functioning associated with somatic and mental health. The most negative impact on the quality of life of the patient induced regularly appearing pain, the fear of health deterioration and unfavorable medical diagnoses during frequent checkups.

Influence of the hemodynamic status of multiorgan donors on long-term kidney graft survival--a multivariable analysis.

Abstract: Background. The quality of transplanted organ and timing of the initiation of its effective function depends on many factors potentially causing graft dysfunction. The aim of this study wastoevaluatethe influence ofthe cardiovascular statusofmultiorgandonors onthelong-term kidney graft survival over a 15-year observation period. Methods. In 2007, the authors of this study published a multicenter prospective study evaluating the influence of the hemodynamic status of multiorgan donors on the early function of transplanted kidney. The results of that study showed that mean arterial pressure, central venous pressure, and pulmonary capillary wedge pressure values of the donor importantly influence the frequency of delayed graft function after renal transplantation. The present analysis covers the effect of the donor’s hemodynamic status parameters on graft function time within the 15-year observation period. Univariate and multivariate analyses using the Cox regression proportional hazard model were performed to evaluate the prognostic parameters for overall survival and renal graft survival time. P < .05 was considered to be significant. Results. The univariate analysis showed a significantly shorter time of graft survival in the group of recipients who had a kidney retrieved from donors with lower pulmonary capillary wedge pressure values (P ¼.038) and lower cardiacindexvalues(P ¼.039).The same results were obtained for the multifactorial Cox logistic regression analysis. Conclusions. The filling of the intravascular bed of the donor as determined by pulmonary capillary wedge pressure and maintained donor tissue perfusion as determined by cardiac index, impose important factors influencing long-term kidney graft survival.

Impact of graft infection on long-term survival after kidney transplant.

Abstract: Background Patients undergoing transplantation procedures are at a high risk of developing infections because of the need for immunosuppression. Infections presenting directly after renal transplantation greatly influence the overall success of the procedure. The aim of this study was to evaluate the influence of postoperative infection on the length of survival after renal transplant. Methods In 2009 a multicenter prospective trial evaluating the factors that influence the occurrence of postoperative infective complications was published by the authors. That study reported that 25 out of 232 recipients of a renal transplant were diagnosed with an infection. The present study shows the effect of postoperative infection on the length of survival after renal transplantation during a 15-year observation period. Statistical methods involved monofactorial and multifactorial Kaplan-Meier analysis for the length of survival and the Cox proportional hazards model for mortality prediction. A P value of Results The analysis demonstrated that the lifespan of renal transplant recipients was decreased in those with postoperative infection, at both year 10 of the observation period ( P = .013) and 15 years after transplantation ( P = .012). Moreover, it was ascertained that an infection in the postoperative period was an independent risk factor increasing the mortality after renal transplantation: P = .026; hazard ratio 2.90 (95% confidence interval, 1.13–7.41). Conclusions The occurrence of an infection in the postoperative period significantly decreases the lifespan of a renal transplantation recipient.

Regional strategies for pro-family policy in the care of rural child

Abstract: Health care of children and young people is part of a system of care of the so called "developmental age population." In this system, prevention plays a key role. The aim of the study was, basing on the obtained results, to develop guidelines for the regional pro-family policy in care of a rural child. The study included children and young people of school age, from 14 to 19 years of age. Overall the analysis included 6,971 children and adolescents and 6,971 parents. Organization of health care of children and school adolescents depended on the solutions approved by individual founding bodies of Health Care Units and Regional Patients' Funds. The withdrawal of nurses from school was observed. The tasks of prevention character performed previously by nurses employed at schools began to be implemented within the framework of primary health care in the child's place of residence. For proper implementation of the tasks of school nurses and the proper development of school hygiene, it is important to establish a uniform and maximum number of pupils per one nurse, taking into account all circumstances, and to develop standards of care for the student, taking into account the age of the student, school type, and the region. Language: en