M
Mark D. Berardini
Researcher at University College London
Publications - 7
Citations - 512
Mark D. Berardini is an academic researcher from University College London. The author has contributed to research in topics: Gel electrophoresis & Oligonucleotide. The author has an hindex of 7, co-authored 7 publications receiving 502 citations.
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Journal ArticleDOI
Rational design of a highly efficient irreversible DNA interstrand cross-linking agent based on the pyrrolobenzodiazepine ring system
D. Subhas Bose,Andrew S. Thompson,Jingshan Ching,John A. Hartley,Mark D. Berardini,Terence C. Jenkins,Stephen Neidle,Laurence H. Hurley,David E. Thurston +8 more
Journal ArticleDOI
An agarose gel method for the determination of DNA interstrand crosslinking applicable to the measurement of the rate of total and "second-arm" crosslink reactions.
TL;DR: The method is illustrated for a number of nitrogen mustard antitumor agents, showing how the moiety attached to a bifunctional reactive group can influence the extent and rate of crosslink formation and, in particular, the conversion of monoadducts to crosslinks.
Journal ArticleDOI
Effect of linker length on DNA-binding affinity, cross-linking efficiency and cytotoxicity of C8-linked pyrrolobenzodiazepine dimers
D. Subhas Bose,Andrew S. Thompson,Melissa Smellie,Mark D. Berardini,John A. Hartley,Terence C. Jenkins,Stephen Neidle,David E. Thurston +7 more
TL;DR: An efficient synthesis of a homologous series of C8-linked pyrrolobenzodiazepine dimers is reported; compounds with an odd number of methylenes in the linker show a higher affinity for DNA, enhanced cross-linking efficiency, and are more cytotoxic compared with compounds with either n= 4 or 6.
Journal ArticleDOI
Alteration in DNA cross-linking and sequence selectivity of a series of aziridinylbenzoquinones after enzymatic reduction by DT-diaphorase.
Chong Soon Lee,John A. Hartley,Mark D. Berardini,John Butler,David Siegel,David Ross,Neil W. Gibson +6 more
TL;DR: DT-diaphorase mediated reduction of a series of 2,5-bis-substituted-3,6-diaziridinyl-1,4-benzoquinones was found to increase the level of DNA interstrand cross-linking (ISC) formed at neutral pH with an enhancement observed as the pH was decreased to 5.8.
Journal ArticleDOI
DNA cross-linking and sequence selectivity of aziridinylbenzoquinones: a unique reaction at 5'-GC-3' sequences with 2,5-diaziridinyl-1,4-benzoquinone upon reduction.
John A. Hartley,Mark D. Berardini,Mauro Ponti,Neil W. Gibson,Andrew S. Thompson,David E. Thurston,Brigid M Hoey,John Butler +7 more
TL;DR: The compounds reacted with all guanine-N7 positions in DNA with a sequence selectivity similar to other chemotherapeutic alkylating agents, such as the nitrogen mustards, although some small differences were observed with BZQ.