Mary Ellen Pusateri

TL;DR: Great diversity was found with regard to both enzyme activities and metabolite levels among individual tumors, but the following generalities can be made.

TL;DR: Methods for 2-deoxyglucose (2-DG) and DG6P are described which are based on the fact that DG6P is oxidized by glucose-6-phosphate dehydrogenase, but at a rate 1000-fold slower than for glucose 6-ph phosphate, whereas hexokinase phosphorylates 2DG and glucose at comparable rates.

TL;DR: The enzymatic methods previously described for DG and 2-deoxyglucose 6-phosphate have been refined and adapted to measurements of brain samples ranging from 50 mg wet weight to less than a microgram dry weight, with emphasis on information useful for employing DG to assess rapid changes in glucose metabolism.

TL;DR: The pattern of Asp distribution suggests that Asp enters brain via fenestrated capillaries serving certain portions of CVO and then spreads into adjacent brain tissue, which supports a growing body of evidence that CVO are an important communication link between blood and brain.

TL;DR: The method is applicable to very small brain samples and illustrated by changes in phosphorylation within 2 min after injection of a convulsant or an anesthetic and over a 48-min time course with and without anesthesia.