Showing papers in "Analytical Biochemistry in 1990"

TL;DR: By combining the least complicated and expedient methods of sample handling with the sensitivity and specificity of the GSH assay by enzymatic recycling and the small volumes and software capabilities of microtiter plate technology, this work devised a rapid, sensitive, and easy assay for GSH and GSSG in biological samples.

TL;DR: The advantage of this method versus one-step zero-length crosslinking is that only one component of the complex is exposed to the crosslinker, which eliminates complications arising from the formation of crosslinks among several proteins of a multicomponent complex.

TL;DR: All assays utilize the Cobas FARA clinical automated analyzer and provide considerable time savings over the manual assays.

TL;DR: The adequacy of thelinear model is investigated, paying special attention to the estimation of the error in the secondary structure estimates, and it is shown that the linear model is only adequate for the alpha-helix class.

TL;DR: Evidence is presented to support a proposed mechanism that oxidative formation of these compounds involves the formation of endoperoxide intermediates which are directly reduced by naturally occurring biological substances to PGF2 compounds.

TL;DR: This derivatization method produced chemically stable substrates which may be useful in studying receptor-mediated cell adhesion, as the quantity of peptide available at the surface may be precisely measured and controlled.

TL;DR: In this article, a simple bisbenzimidazole (Hoechst 33258) was used to determine cellular DNA content in 96-well tissue cultures plates.

TL;DR: There was good agreement between cAMP values obtained with the protein-binding method and commercially available radioimmunoassays that were used as reference methods.

TL;DR: This filter paper assay is useful for determining 100 ng to 20 micrograms of protein in the presence of ammonium sulfate, urea, thiol-reducing agents, amino acids, DNA, ionic and nonionic detergents, and acid or base.

TL;DR: A rapid temperature cycler of low thermal mass was constructed and a 536-bp beta-globin fragment of human genomic DNA was easily visualized with ethidium bromide on agarose gels with rapid cycling.

TL;DR: The convenience of the ferricenium method suggests it may be generally useful as a screening assay for a number of acyl-CoA dehydrogenases, and avoids the necessity of purifying substantial amounts of electron transferring flavoprotein.

TL;DR: The specific reaction of monochlorobimane with GSH is used to analyze hepatic GSH and determine directly the rate of synthesis of GSH in both cell-free conditions and in cell suspensions by monitoring the increase in fluorescent adduct when mBC1 is present in excess in the incubation.

TL;DR: A simplified method is described here for isolating the recombinant Taq enzyme after overproduction in Escherichia coli and contains a single, nearly homogeneous protein consistent with the previously established size of the Taq DNA polymerase.

TL;DR: This method will allow the identification of chemical components, receptors, or ionic channels present in a specific type of cell, to determine their relevance to the regulation of the differential secretion of specific materials present in one but not in the other cell type and to ascertain whether the released materials from one cell type affect the functions of the other.

TL;DR: This method has been successfully applied to the isolation and purification of DNA from eight different adult insects and can be cleaved with restriction endonucleases, ligated efficiently using standard cloning vectors, and hybridized to synthetic oligonucleotides.

TL;DR: A dimethylbarbituric acid reagent has been used to follow the kinetics of loss of two water-soluble carbodiimides, 1-ethyl-3-(3-dimethylaminopropyl) Carbodiimide (EDC) and the structurally related EAC, in aqueous solution as a function of pH and added chemical reagents.

TL;DR: Radioisotopic assays for the determination of acetyl-CoA, CoASH, and acetylcarnitine have been modified for application to the amount of human muscle tissue that can be obtained by needle biopsy.

TL;DR: A spectrophotometric method is described for the determination of sarcoplasmic reticulum (SR) Ca2(+)-ATPase activity (EC 3.1.6.38) in unfractionated muscle homogenates, which shows a good correlation over a wide range between SR specific Ca2 (+)-uptake and -ATPases activities.

TL;DR: To characterize the phenomenon, a series of PCR primers were made and whether differential amplification could be detected after agarose gel electrophoresis was determined and the following conclusions emerge.

TL;DR: The assay is characterized by a sensitivity sufficiently high to detect the various forms of glutathione in plasma, by an analytical recovery of GSH and GSSG close to 100%, and by a within-day precision corresponding to a coefficient of variation of 7%.

TL;DR: Overnight electrophoresis was replaced by short gel runs and overnight capillary transfer by rapid vacuum-blotting adapted to Northern analysis and the duration of the procedure was shortened drastically, allowing an autoradiography signal to be obtained within 24 h.

TL;DR: A quantitatively similar degree of protein yields was obtained for the beta-galactoside-binding protein of bovine heart, although different proteins were obtained when neoglycoproteins were used as ligand.

TL;DR: The length distribution of the glycan strands in the murein (peptidoglycan) sacculus of Escherichia coli has been analyzed after solubilization of the muresin by complete digestion with human serum amidase.

TL;DR: A method where lectins conjugated with digoxigenin are used in combination with an anti-digoxigen in antibody AP conjugate as a very sensitive detection system for carbohydrate structures analysis of blotted glycoproteins is described.

TL;DR: This assay relies on the use of a large excess of a low affinity ligand which removes and complexes all low molecular weight iron and iron nonspecifically bound to serum proteins.

TL;DR: A quantitative assay for human immunodeficiency virus type 1 (HIV-1) proviral DNA sequences using the polymerase chain reaction (PCR) and the procedure described here is generally applicable to the quantitation of other retroviruses.

TL;DR: It is shown that the polymerase chain reaction is a very efficient technique for synthesis of digoxigenin-labeled DNA and an extremely simple procedure for purification of the non-isotopically labeled fragments is presented.

TL;DR: A new method for the separation of sialic acids at neutral pH on a Carbopac PA-1 anion-exchange column of pellicular resin, with pulsed amperometric detection following postcolumn addition of alkali is reported.