M
Masatoshi Yamaguchi
Researcher at Fukuoka University
Publications - 248
Citations - 5164
Masatoshi Yamaguchi is an academic researcher from Fukuoka University. The author has contributed to research in topics: Derivatization & High-performance liquid chromatography. The author has an hindex of 36, co-authored 244 publications receiving 4982 citations. Previous affiliations of Masatoshi Yamaguchi include Chugai Pharmaceutical Co. & Kyushu University.
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Journal ArticleDOI
Fluorometric high-performance liquid chromatography of N-acetyl- and N-glycolylneuraminic acids and its application to their microdetermination in human and animal sera, glycoproteins, and glycolipids.
TL;DR: A simple, rapid, and highly sensitive high-performance liquid chromatographic method is described for the determination of N-acetyl- and N-glycolylneuraminic acids in human and animal sera, glycoproteins, and glycolipids.
Journal ArticleDOI
Determination of mono-O-acetylated N-acetylneuraminic acids in human and rat sera by fluorometric high-performance liquid chromatography.
TL;DR: A simple, rapid, and highly sensitive fluorometric high-performance liquid chromatographic method for the determination of N- acetylneuraminic acid and its mono-O-acetyl derivatives in human and rat sera is described.
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Highly Selective Fluorometric Determination of Polyamines Based on Intramolecular Excimer-Forming Derivatization with a Pyrene-Labeling Reagent
Hitoshi Nohta,Hiroshi Satozono,Katsumi Koiso,Hideyuki Yoshida,Junichi Ishida,Masatoshi Yamaguchi +5 more
TL;DR: The present method was so selective that biogenic monoamines gave no peak in the chromatogram and the structures of the derivatives were confirmed by HPLC with mass spectrometry, and the emission of excimer fluorescence could be proved by spectrofluorometry and time-resolved fluorometry.
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Highly sensitive determination of N-acetyl- and N-glycolylneuraminic acids in human serum and urine and rat serum by reversed-phase liquid chromatography with fluorescence detection.
TL;DR: The method was applied to the determination of the neuraminic acids in sera from normal subjects and cancer patients, normal urine and rat serum, where the limit of detection is 40 fmol (12 pg) for both neuramini acids in 10-microliters injection volume.
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Determination of serotonin, noradrenaline, dopamine and their metabolites in rat brain extracts and microdialysis samples by column liquid chromatography with fluorescence detection following derivatization with benzylamine and 1,2-diphenylethylenediamine.
TL;DR: The new fluorescence derivatization protocol provides an excellent means for simultaneous determination of all three monoamines both in the complex samples (e.g. brain homogenates) and also at trace levels, such as those found in the microdialysis samples.