M
Masayoshi Sakaguchi
Researcher at Kogakuin University
Publications - 38
Citations - 692
Masayoshi Sakaguchi is an academic researcher from Kogakuin University. The author has contributed to research in topics: Chitinase & Chitin. The author has an hindex of 14, co-authored 38 publications receiving 545 citations.
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Journal ArticleDOI
Acidic mammalian chitinase is a proteases-resistant glycosidase in mouse digestive system
Misa Ohno,Masahiro Kimura,Haruko Miyazaki,Kazuaki Okawa,Riho Onuki,Chiyuki Nemoto,Eri Tabata,Satoshi Wakita,Akinori Kashimura,Masayoshi Sakaguchi,Yasusato Sugahara,Nobuyuki Nukina,Nobuyuki Nukina,Nobuyuki Nukina,Peter O. Bauer,Fumitaka Oyama +15 more
TL;DR: Evidence is provided that acidic mammalian chitinase (AMCase) can function as a protease-resistant major glycosidase under the conditions of stomach and intestine and degrade chit in substrates to produce (GlcNAc)2, a source of carbon, nitrogen and energy.
Journal ArticleDOI
Chitin digestibility is dependent on feeding behaviors, which determine acidic chitinase mRNA levels in mammalian and poultry stomachs.
Eri Tabata,Akinori Kashimura,Azusa Kikuchi,Hiromasa Masuda,Ryo Miyahara,Yusuke Hiruma,Satoshi Wakita,Misa Ohno,Masayoshi Sakaguchi,Yasusato Sugahara,Vaclav Matoska,Peter O. Bauer,Fumitaka Oyama +12 more
TL;DR: The results indicate that feeding behavior affects Chia expression levels as well as chitinolytic activity of the enzyme, and determines chitin digestibility in the particular animals.
Journal ArticleDOI
Gastric and intestinal proteases resistance of chicken acidic chitinase nominates chitin-containing organisms for alternative whole edible diets for poultry
Eri Tabata,Akinori Kashimura,Satoshi Wakita,Misa Ohno,Masayoshi Sakaguchi,Yasusato Sugahara,Yoshihiro Kino,Vaclav Matoska,Peter O. Bauer,Fumitaka Oyama +9 more
TL;DR: Functional similarity of chicken Chia with the mouse enzyme suggests that chitin-containing organisms can be used for alternative poultry diets not only as whole edible resources but also as enhancers of their nutritional value.
Journal ArticleDOI
Quantification of Chitinase mRNA Levels in Human and Mouse Tissues by Real-Time PCR: Species-Specific Expression of Acidic Mammalian Chitinase in Stomach Tissues.
Misa Ohno,Yuto Togashi,Kyoko Tsuda,Kazuaki Okawa,Minori Kamaya,Masayoshi Sakaguchi,Yasusato Sugahara,Fumitaka Oyama +7 more
TL;DR: A quantitative PCR system was developed to compare the mRNA levels between human and mouse tissues using a human-mouse hybrid standard DNA and showed that Chit1 mRNA is expressed at similar levels in normal human andmouse lung, whereas AMCase mRNA was not overexpressed innormal human stomach tissues.
Journal ArticleDOI
Protein A-mouse acidic mammalian chitinase-V5-His expressed in periplasmic space of Escherichia coli possesses chitinase functions comparable to CHO-expressed protein.
Akinori Kashimura,Kazuaki Okawa,Kotarou Ishikawa,Yuta Kida,Kokoro Iwabuchi,Yudai Matsushima,Masayoshi Sakaguchi,Yasusato Sugahara,Fumitaka Oyama +8 more
TL;DR: The E. coli-expressed Protein A-mouse AMCase-V5-His fusion protein possesses chitinase functions comparable to the CHO-expressive AMCase and can be used to elucidate detailed biomedical functions of the mouse AMCase.